Full-thickness Skin Harvesting
Full-thickness Skin Harvesting - Related Videos
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
08:35
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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Cited by 53 •
2015
The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.
05:50
CUBIC-based Protein Expression Visualization: A Procedure for Visualizing Protein Expression in Full Thickness Mouse Skin Biopsies
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2023
This video describes the CUBIC-based visualization technique, a tissue clearing-dependent imaging technique for visual assessment of protein expression in whole-mount skin biopsies at single-cell resolution in 3D. This technically simple protocol allows the visualization of skin proteins without preparing skin sections.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
FAQs
After placement, graft success depends on revascularization from the recipient bed. The transplanted tissue must establish a blood supply there so its preserved layers can participate in structural and functional repair. This makes the condition of the recipient site a central determinant of outcome and a key variable when full-thickness grafts are studied as repair materials.
The retained connective-tissue thickness matters because full-thickness harvesting preserves the tissue layers needed for structural and functional repair. In bioengineering studies, that layered architecture helps maintain clinical relevance when investigators examine skin regeneration, wound healing, or the performance of engineered substitutes. The value is therefore not only tissue coverage, but representation of the organization being repaired.
Donor-site management depends on the wound's size and location. A smaller or favorably located defect may be closed primarily, whereas other wounds may require management suited to their dimensions and anatomical position. These variables are part of the harvesting plan because removing tissue creates a separate wound that must be addressed alongside preparation of the graft.
During excision, the operator carefully removes the graft with its full connective-tissue thickness rather than separating away the deeper dermal component. Precision is important because the harvested specimen must retain the layers selected for repair or investigation. This requirement links surgical handling directly to the structural quality and usefulness of the resulting graft.
At a practical level, the workflow has two linked parts: excising the full-thickness graft from a donor site and closing or otherwise managing the donor wound. The graft is then intended for placement on a recipient bed where revascularization can occur. Considering both sites prevents the harvest step from being treated as an isolated tissue-removal procedure.
Bioengineers use full-thickness skin grafts as clinically relevant tissue models and repair materials. They can support investigations of wound healing and skin regeneration, while also providing a reference context for biomaterials and engineered skin substitutes. Their relevance comes from connecting experimental design with the layered tissue requirements encountered in structural and functional repair.
In engineered-skin research, these grafts help frame whether a proposed substitute addresses more than surface coverage. Studies can consider how biomaterials or engineered constructs relate to skin regeneration and wound healing while using a clinically relevant graft context. This supports comparisons between bioengineering experiments and the repair challenges represented by full-thickness tissue.