Time-gated Spectroscopy
Time-gated Spectroscopy - Related Videos
08:53
Dependence of Laser-induced Breakdown Spectroscopy Results on Pulse Energies and Timing Parameters Using Soil Simulants
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Cited by 3 •
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LIBS detection capabilities on soil simulants were tested using a range of pulse energies and timing parameters. Calibration curves were used to determine detection limits and sensitivities for different parameters. Generally, the results showed that there was not a significant reduction in detection capabilities using lower pulse energies and non-gated detection.
12:55
P50 Sensory Gating in Infants
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Cited by 5 •
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Methods to record auditory P50 sensory gating, a physiological marker of cerebral inhibition which reflects early stages of attention, are described.
10:00
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
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Cited by 12 •
2014
Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.
09:21
Mössbauer Spectroscopy
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2023
Source: Joshua Wofford, Tamara M. Powers, Department of Chemistry, Texas A&M University Mössbauer spectroscopy is a bulk characterization technique that examines the nuclear excitation of an atom by gamma rays in the solid state. The resulting Mössbauer spectrum provides information about the oxidation state, spin state, and electronic environment around the target atom, which, in combination, gives evidence about the electronic structure and ligand arrangement (geometry) of the molecule.
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
FAQs
The delay determines how long the detector waits after the excitation pulse, while the gate width determines how long emission is collected. Increasing the delay can reduce prompt scattering and short-lived background, whereas changing the gate width alters how much remaining emission contributes to the signal. These settings therefore influence background suppression and the measured intensity.
Autofluorescence and other background components may decay differently from the emission of a molecular probe or engineered material. By opening detection after excitation and selecting an appropriate collection interval, the measurement can reduce contributions from prompt scattering and short-lived background. This improves the visibility of longer-lived target emission in cells, tissues, biomaterials, and related samples.
Emission intensity reports how much signal is detected, whereas lifetime describes how the signal decays after excitation. Recording both can help distinguish emissions that overlap spectrally but behave differently over time. In bioengineering studies, these measurements can support characterization of molecular probes, sensing responses, biomaterials, and engineered biological systems when intensity alone is difficult to interpret.
A typical measurement begins by exciting the sample with a pulsed light source. The detector is then configured with a defined delay and gate width, so it records emission only during the selected interval. Researchers collect the gated signal and may examine its intensity or decay behavior to assess background suppression and characterize the sample’s optical response.
The pulsed excitation source, timing control, detector, delay, and gate width must operate together. The source establishes the reference excitation event, while electronic or mechanical control determines when detection begins and ends. Coordinating these elements is essential because the selected timing controls which portions of the emission decay are included in the recorded measurement.
Time-gated spectroscopy is relevant when researchers need optical measurements from cells, tissues, biomaterials, molecular probes, or engineered biological systems in the presence of interfering background. Its ability to record selected portions of emission can support sensing, imaging, assay development, and sample characterization. The resulting intensity and lifetime information can help evaluate biological or engineered-system signals.