Vascular Infiltration
Vascular Infiltration - Related Videos
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
04:00
Immunohistochemistry to Study the Relationship Between Macrophages and Infiltrated Immune Cells
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2025
This video demonstrates a technique to quantify the relative ratio of macrophages among the total infiltrated immune cell populations in temporal artery biopsy. The immunohistochemical staining of a tissue section affected by autoimmune disease selectively stains the immune cells with specific receptors to visually differentiate them from the rest of the cells.
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
14:24
Manufacturing of Three-dimensionally Microstructured Nanocomposites through Microfluidic Infiltration
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Cited by 4 •
2014
Three-dimensional (3D) microstructured composite beams are fabricated through the directed and localized infiltration of nanocomposites into 3D porous microfluidic networks. The flexibility of this manufacturing method enables the utilization of different thermosetting materials and nanofillers in order to achieve a variety of functional 3D reinforced nanocomposite macroscopic products.
08:22
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
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Cited by 37 •
2017
We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.
FAQs
These factors regulate how readily endothelial cells migrate and form sprouts within a construct. Scaffold structure can provide the physical environment for penetration, while cell signals guide vascular behavior. Oxygen gradients also influence the developing network, helping determine where infiltration proceeds and whether the construct can establish effective exchange with surrounding tissue.
A typical sequence begins with endothelial cell migration into the target tissue or biomaterial, followed by sprouting of new vascular extensions. These sprouts then develop lumens, meaning internal spaces capable of supporting flow, and may connect with pre-existing vessels. Completion of this sequence supports exchange between the engineered construct and the surrounding vascular network.
Lumen formation marks a transition from cellular penetration to development of a more organized vascular structure. The lumen provides the internal space needed for exchange within the emerging network and helps support connection with existing vessels. Without progression beyond migration and sprouting, infiltration may not provide the functional transport needed by engineered tissue.
Designers can account for infiltration by coordinating scaffold structure, cellular signaling, and oxygen conditions within the construct. These features should support endothelial migration, sprouting, lumen formation, and eventual vascular connection. Considering the sequence during construct design can improve access to oxygen and nutrients while promoting removal of metabolic waste from developing tissue.
Controlled vascular infiltration is particularly relevant to tissue-engineered grafts, organoids, and implants. In each case, developing tissue may require improved access to oxygen and nutrients as well as removal of metabolic waste. Better vascular integration can support construct survival and maturation, making it important for regenerative medicine approaches aimed at functional restoration.
A useful outcome is improved vascular integration between the construct and surrounding tissue, accompanied by more effective exchange. This integration can help maintain construct survival, promote maturation, and support functional restoration. In bioengineering, these outcomes connect the cellular steps of infiltration with the broader goal of producing viable, functioning grafts, organoids, or implants.