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Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
JoVE Journal
Medicine
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JoVE Journal Medicine
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

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07:49 min

April 13, 2015

DOI:

07:49 min
April 13, 2015

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Summary

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This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.

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