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Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
 

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Article doi: 10.3791/52657
April 13th, 2015

Summary April 13th, 2015

This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.

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