Cytidine Deaminase
Cytidine Deaminase - Related Videos
04:17
Determining the Serum Stability of Human Adenosine Deaminase 1 Enzyme
0 Views •
2024
In this article, we detail methods to characterize an enzyme's ability to retain function when incubated at 37 °C in human serum, a pharmacological property referred to as its serum stability. This ability may be a key factor in predicting an enzyme's pharmacokinetic profile and its suitability for therapeutic use.
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
0 Views •
2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
02:23
RNA Editing
0 Views •
2020
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
06:26
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
0 Views •
Cited by 5 •
2012
Described is a two-step labeling process using β-glucosyltransferase (β-GT) to transfer an azide-glucose to 5-hmC, followed by click chemistry to transfer a biotin linker for easy and density-independent enrichment. This efficient and specific labeling method enables enrichment of 5-hmC with extremely low background and high-throughput epigenomic mapping via next-generation sequencing.
02:57
Transfecting Primary Macrophages with Modified mRNA
0 Views •
2025
This video demonstrates the process of transfecting primary macrophages with modified mRNA encoding green fluorescent protein. These modified mRNAs, designed to reduce immunogenicity and improve its stability, ensure the successful transfection and subsequent expression of green fluorescent proteins, which is confirmed through fluorescence microscopy.
FAQs
Catalysis depends on a conserved active site that positions zinc and water for removal of cytidine’s amino group. The resulting uridine can alter nucleic acid sequence information, but the biological consequence depends on the substrate being examined. This catalytic arrangement explains why related enzymes can support distinct RNA and DNA studies.
Substrate recognition determines whether an enzyme acts on RNA, single-stranded DNA, or both. That selectivity directs the type of sequence information affected and shapes the experimental interpretation. RNA-targeting activity can support editing studies, whereas activity on single-stranded DNA can inform investigations of mutagenesis or other DNA-associated biological functions.
Hydrolytic deamination changes nucleic acid sequence information by replacing cytidine-derived information with uridine-derived information. In biological research, that chemical change provides a basis for examining RNA editing, DNA mutagenesis, antibody diversification, and host defense. The same reaction therefore connects molecular catalysis with several distinct cellular and organismal processes.
A practical workflow begins by matching the enzyme’s substrate preference to the question: an RNA-directed study, a single-stranded DNA study, or an investigation involving both. Researchers then use the resulting sequence changes to examine RNA editing, DNA mutagenesis, antibody diversification, or host defense. This alignment connects catalytic activity with the biological function under study.
Engineered deaminases supply the catalytic component of base editors, which direct cytidine deamination toward targeted sequence changes. Depending on the editor design, this approach can produce C-to-T or C-to-G changes without creating double-strand DNA breaks. It therefore differs from methods that rely on breaking both DNA strands to alter sequence information.
Applications extend across RNA and DNA biology. Researchers can use relevant deaminase activities to investigate RNA editing, DNA mutagenesis, antibody diversification, and host defense, while substrate specificity helps distinguish which molecular context is being studied. This broad range makes the enzyme useful for connecting biochemical activity with biological techniques and functional questions.