Dry Mass
Dry Mass - Related Videos
08:07
Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
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Cited by 1 •
2025
This protocol aims to preserve cell metabolites for single-cell mass spectrometry (SCMS) by combining volatile salt solution washing, rapid liquid nitrogen quenching, freeze-drying, and −8 °C storage. It demonstrates that liquid nitrogen quenching is essential for maintaining metabolite profiles, while prolonged cold storage should be minimized to prevent metabolic alterations.
01:30
Dry Friction
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2023
Dry friction occurs between two solid surfaces in contact as they attempt to move relative to one another. In daily life, dry friction is encountered in various forms, such as when walking on the ground, sliding an object across a table, or rubbing hands together. Despite its ubiquity, the underlying mechanisms behind dry friction are not readily visible. To illustrate this concept, imagine a wooden crate resting on a rough, non-uniform horizontal surface. When an external force is applied to...
08:38
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
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Cited by 29 •
2015
Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.
01:21
Drying Shrinkage
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2024
When hardened concrete is exposed to air with a relative humidity of less than 100 percent, it begins to lose the free water within its capillaries. As this water evaporates, the water initially adsorbed onto the calcium silicate hydrates migrates towards these now empty spaces and eventually evaporates as well. Over time, as more water leaves, the volume of the concrete decreases, a phenomenon known as drying shrinkage. A portion of this drying shrinkage can be reversed; if the concrete is...
10:23
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
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Cited by 7 •
2017
Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.
FAQs
Fresh mass can vary substantially because it includes changing amounts of water, even when the amount of cellular material is similar. Dry mass reduces that source of variation, allowing comparisons to focus more directly on solid biomass. This makes it useful when assessing growth or biomass production across samples or experimental conditions.
Drying until mass becomes stable provides a consistent endpoint for weighing. Before that point, residual water can continue to influence the measured value, making samples difficult to compare. Controlled conditions help separate the drying process from biological differences, so the resulting measurement more reliably reflects changes in solid material between samples.
When samples undergo comparable preparation and drying, a difference in dry mass indicates a difference in the amount of remaining solid biological material. This interpretation is valuable because water content can change independently of cellular material. Researchers can therefore distinguish apparent changes caused by hydration from changes associated with biomass across experimental conditions.
Researchers first obtain a biological sample, remove its water under controlled drying conditions, and continue the process until the remaining mass is stable. They then weigh the residue and use that value for comparison. Applying the same workflow to samples makes dry mass suitable for tracking changes across experimental conditions.
Dry mass measurements apply across several biological systems, including microorganisms, plants, and algae. In each case, the measurement supplies a common way to quantify biological material, despite major differences in organismal form. This broad applicability supports comparisons of growth and biomass production across studies focused on different types of organisms.
By measuring the solid material present under defined experimental conditions, researchers can compare biomass production and assess how biological material changes. Those comparisons help investigate growth and resource allocation, linking a measured mass outcome to broader biological questions about how organisms accumulate material during an experiment.