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JoVE Journal
Biochemistry

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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
 

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Article doi: 10.3791/55585
April 17th, 2017

Summary April 17th, 2017

Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.

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