Heart-specific Promoter
Heart-specific Promoter - Related Videos
10:58
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
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Cited by 15 •
2017
Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.
12:09
Patient-specific Modeling of the Heart: Estimation of Ventricular Fiber Orientations
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Cited by 14 •
2013
A methodology to estimate ventricular fiber orientations from in vivo images of patient heart geometries for personalized modeling is described. Validation of the methodology performed using normal and failing canine hearts demonstrate that that there are no significant differences between estimated and acquired fiber orientations at a clinically observable level.
02:40
The Eukaryotic Promoter Region
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2020
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
09:06
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
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Cited by 3 •
2016
Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).
07:27
Implantation of Total Artificial Heart in Congenital Heart Disease
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Cited by 40 •
2014
This is a case report of a patient with congenitally corrected transposition of the great arteries (CCTGA) who received a total artificial heart (TAH) as a bridge to heart transplant. The TAH was successfully implanted with modifications to accommodate the patient's congenitally malformed heart.
FAQs
Cardiac transcription factors bind the promoter or nearby regulatory elements and help recruit RNA polymerase II, connecting DNA recognition with transcription initiation. This recruitment does not act independently of the surrounding genome. Chromatin state and cellular context can influence whether transcription proceeds efficiently, meaning that factor binding alone does not fully determine expression in cardiac cells.
A heart-specific promoter responds not only to its sequence but also to the chromatin environment and cellular context in which it operates. These conditions can change how strongly transcription occurs and when it begins or declines. Consequently, a promoter that performs well in one model may show different activity in another, requiring model-specific evaluation.
These promoters can favor expression in cardiac cells and reduce expression in noncardiac tissues, but their performance still depends on the biological model. Uneven activity may affect how researchers interpret a reporter signal or the distribution of a transgene. Validation in each model is therefore important before attributing an observed effect exclusively to cardiac expression.
Researchers can use the promoter to drive a reporter gene, a gene-editing component, or a therapeutic transgene. The selected payload determines what the experiment measures or changes, while the promoter provides preferential cardiac control. After constructing the system, researchers should evaluate its performance in the intended model rather than assuming that activity will remain consistent across settings.
They are useful when investigators need to examine or manipulate cardiac biology during heart development, disease, or regeneration. A reporter can help study expression patterns, whereas gene-editing components or therapeutic transgenes can support targeted experimental manipulation. Preferential activity in cardiac cells can make conclusions easier to interpret by limiting expression in noncardiac tissues.
Researchers should assess how strongly and selectively the promoter functions in the specific model, including whether expression appears in cardiac cells and is limited in noncardiac tissues. They should also consider the intended payload and experimental context. This validation helps determine whether the promoter is appropriate for reporting, gene editing, or therapeutic transgene expression.