Pancreatic Bud Dissection
Pancreatic Bud Dissection - Related Videos
08:04
Pancreatic Tissue Dissection to Isolate Viable Single Cells
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Cited by 5 •
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Pancreatic metaplastic cells are precursors of malignant cells that give rise to pancreatic tumors. However, isolating intact viable pancreatic cells is challenging. Here, we present an efficient method for pancreatic tissue dissociation. The cells can then be used for single-cell RNA sequencing (scRNA-seq) or for two- or three-dimensional co-culturing.
05:11
Treatment of Middle-segment Pancreatic Benign Tumor Using Laparoscopic Central Pancreatectomy with End-to-end Pancreatic Duct Reconstruction
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This article presents laparoscopic central pancreatectomy with pancreatic duct end-to-end anastomosis as an important surgical approach for middle-segment pancreatic benign and low-grade malignant tumors.
11:54
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
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Cited by 20 •
2009
Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part I: digestion and collection of pancreatic tissue) using a modified automated method.
09:25
Bioluminescent Orthotopic Model of Pancreatic Cancer Progression
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Cited by 34 •
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Improved understanding of pancreatic cancer biology is critically needed to enable the development of better therapeutic options to treat pancreatic cancer. To address this need, we demonstrate an orthotopic model of pancreatic cancer that permits non-invasive monitoring of cancer progression using in vivo bioluminescence imaging.
00:49
The Tongue and Taste Buds
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2025
The surface of the tongue is covered with various small bumps called papillae, which either distribute what has been ingested (filiform papillae) or contain the sensory taste (or gustatory) receptor cells (fungiform, circumvallate, and foliate papillae). Embedded within each taste-related papilla are the taste buds—clusters of 30 to 100 gustatory receptor cells. Gustatory receptor cells extend finger-like projections called gustatory hairs (or microvilli) into a region known as the taste pore.
FAQs
Branching morphogenesis can be observed as pancreatic epithelial cells organize and extend branches while the isolated tissue remains in culture. This provides a direct way to study how developing pancreatic structure emerges over time, rather than examining only a finished organ. Researchers can therefore connect tissue architecture with the signals regulating organ formation.
Cell-cell interactions, growth factors, and other tissue signals help regulate pancreatic development within the cultured bud. Studying these influences separately or together allows investigators to examine how local communication affects epithelial behavior, branching, and differentiation. The approach is therefore useful for testing molecular pathways that may control normal organ formation.
The isolated developing tissue allows researchers to follow how pancreatic cells differentiate toward endocrine or exocrine lineages in a controlled environment. Comparing these developmental outcomes with the signals and interactions present in culture can reveal relationships between molecular regulation and cell fate. This supports broader analysis of how distinct pancreatic cell populations arise.
A typical workflow begins with locating the developing pancreatic tissue in an embryo under a stereomicroscope. Researchers then carefully separate the pancreatic bud from surrounding tissues and transfer the isolated material into culture. Subsequent observation focuses on branching morphogenesis, epithelial differentiation, and responses to the developmental conditions being studied.
Culture provides a controlled environment in which investigators can directly examine tissue development after isolation. They can observe epithelial branching and differentiation while evaluating how cell-cell interactions, growth factors, or other tissue signals influence those outcomes. This makes the preparation useful for linking experimental conditions with visible changes in pancreatic organization and cell development.
This technique is especially useful when researchers need to analyze pancreatic organ formation, test molecular pathways, or investigate endocrine and exocrine differentiation. It also contributes to models of congenital disorders and pancreatic regeneration by providing a developing tissue system in which regulatory signals and developmental responses can be examined directly.