Pbs Dissection
Pbs Dissection - Related Videos
10:18
Dissection of Organs from the Adult Zebrafish
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Cited by 168 •
2010
This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
07:40
Dissection of the Adult Zebrafish Kidney
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Cited by 38 •
2011
The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.
06:29
Dissection of Larval CNS in Drosophila Melanogaster
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Cited by 18 •
2006
In this article we demonstrate how to dissect the central nervous system from third instar Drosophila larvae.
08:30
Dissection and Culture of Mouse Embryonic Kidney
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Cited by 4 •
2017
This protocol describes a method for isolating and culturing metanephric rudiments from mouse embryos.
07:37
The Gateway to the Brain: Dissecting the Primate Eye
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Cited by 7 •
2009
The non-human primate is an important translational species for our understanding of development and aging. The anatomical organization of the primate retina may provide important insights into normal and pathological conditions in humans.
FAQs
An isotonic solution has a solute balance that helps limit osmotic stress while tissue is being isolated. The phosphate buffer also helps maintain a near-physiological aqueous setting, so specimens are less likely to experience handling-related changes caused by drying or inappropriate solution conditions. This matters when comparing tumor samples prepared for later analyses.
PBS does not replace a fixative because its role is to provide a buffered, isotonic environment during isolation rather than preserve cellular structure. This distinction determines how specimens are handled afterward: PBS preparation supports collection for later work, while structural preservation requires an appropriate fixative when that is the experimental goal.
Rinsing tissue with PBS helps remove blood and debris that could remain on the specimen surface. This improves tissue cleanliness, while hydration and reduced handling artifacts can support more consistent preparation. These benefits become especially relevant when samples from different tumors or experimental groups must be compared using histology, imaging, molecular assays, or cell-based studies.
During specimen preparation, investigators use PBS while isolating the tumor and any adjacent tissue, rinse away blood and debris, and keep the specimens hydrated during handling. The collected material can then be directed to the downstream assay selected for the study. Consistent execution helps reduce handling artifacts across samples and supports more reliable comparisons.
Material prepared with this approach may be directed to histology, imaging, molecular assays, or cell-based studies. The suitable downstream use depends on what the experiment needs from the specimen, because PBS helps with cleanliness and hydration but does not preserve cellular structure as a fixative would. It therefore supports multiple cancer-research workflows without replacing structural preservation methods.
Collecting tumors together with adjacent tissues gives cancer researchers a consistent way to prepare the specimen types needed for comparative studies. Using PBS across these samples can help keep handling conditions similar, remove blood and debris, and limit osmotic damage. Those controls support cleaner interpretation when researchers compare tissues through histology, imaging, molecular assays, or cell-based studies.