Carbon Disulfide Adduct
Carbon Disulfide Adduct - Related Videos
03:51
Fluorescent Functionalization of Virus-Like Particles via Disulfide Re-bridging
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2026
Source: Chen, Z., et al. Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry. J. Vis. Exp. (2018)This video demonstrates the fluorescent functionalization of virus-like particles by re-bridging reduced disulfides with a polyethylene glycol (PEG) linker, producing stable, trackable conjugates suitable for cellular imaging and targeted delivery in biomedical applications.
07:52
Removal of Branched and Cyclic Compounds by Urea Adduction for Uk'37 Paleothermometry
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst As mentioned in previous videos, the product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds. In the case of our two organic paleothermometers (Uk'37 and MBT/CBT), the interest is in only 6 compounds (2 alkenones and 4 isoprenoidal glycerol dialkyl glycerol...
11:46
Single Molecule Analysis of Laser Localized Psoralen Adducts
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2017
Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.
10:18
Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry
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Cited by 12 •
2018
Here, we present a procedure to fluorescently functionalize the disulfides on Qβ VLP with dibromomaleimide. We describe Qβ expression and purification, the synthesis of dibromomaleimide-functionalized molecules, and the conjugation reaction between dibromomaleimide and Qβ. The resulting yellow fluorescent conjugated particle can be used as a fluorescence probe inside cells.
02:44
Non-Reducing SDS PAGE: A Method to Analyze Disulfide-Linked Multimeric Protein Complexes
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2025
This video describes the technique of separating the protein samples by non-reducing SDS-PAGE to analyze the multimeric protein complexes. This technique retains the multimer subunits of a protein held by the disulfide bonds which can later be analyzed by western blotting.
FAQs
The carbon atom of CS₂ acts as the electrophilic site, so an electron-rich nucleophile directs bond formation there. This initial attack changes how charge is distributed across the molecule, while the sulfur atoms help stabilize the resulting charged structure. Recognizing this polarity provides a mechanistic basis for predicting which part of CS₂ reacts first.
Sulfur atoms accommodate and stabilize charge generated after nucleophilic attack on CS₂. They can also participate in coordination or undergo further reaction, giving the resulting species additional pathways beyond the initial interaction. This combination of charge stabilization and sulfur reactivity explains why carbon disulfide adducts can serve as intermediates in broader sulfur-containing chemical transformations.
The identity of the reacting partner strongly influences the adduct's structural class. Reactions involving suitable organic or ionic partners can produce dithiocarbonate or dithiocarbamate motifs, whereas interaction with a metal center can generate a metal-associated structure. These categories therefore reflect differences in bonding partners and coordination, not merely different names for one identical species.
Reaction conditions help determine which type of interaction develops after CS₂ encounters its partner. The overview identifies conditions, together with the reacting partner, as factors that influence whether dithiocarbonate, dithiocarbamate, or metal-associated structures arise. Comparing products under different conditions can therefore reveal how bonding, charge stabilization, and subsequent sulfur reactivity are connected.
Analysis should focus on the identity of the reacting partner, the site of new interaction, and the role of the sulfur atoms after nucleophilic attack. It is also important to determine whether the product contains a dithiocarbonate, dithiocarbamate, or metal-associated arrangement. This framework connects molecular structure with the adduct's likely reactivity and coordination behavior.
These adducts support several areas of chemistry, including organic synthesis, inorganic and coordination chemistry, and reaction mechanism analysis. Their sulfur-containing structures also contribute to the design of sulfur-containing materials. Studying how CS₂ bonds to nucleophiles or metal centers helps researchers relate molecular formation processes to synthetic strategies, coordination behavior, and material-oriented chemical design.