Lactic Acid
Lactic Acid - Related Videos
03:24
Revival and Growth of Lactic Acid Bacteria
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2025
Source: Ayivi, R. et al., The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective. J. Vis. Exp. (2022)This video demonstrates the method for recovering cold-stressed lactic acid bacteria from a frozen glycerol stock, restoring bacterial viability through anaerobic incubation, and isolating active colonies for culture expansion.
11:28
High-Throughput Screening of L-Lactic Acid-Hyperproducing Bacillus coagulans Mutants Using an Automated Droplet Microfluidic Platform
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2026
This protocol presents a standard high-throughput screening workflow for industrial microorganisms. It involves constructing a mutant library via ARTP mutagenesis, performing positive droplet screening with automated microfluidics, and subsequently verifying the results. The workflow’s reliability was validated by screening high-yield B. coagulans strains.
04:40
The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
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Cited by 6 •
2022
The quality control assessment of lactic acid bacteria (LAB) cultures has been confirmed as an effective way to enhance the viability and functionality of LAB strains for fermentation procedures. To buttress this assertion, we developed a protocol that elucidates how LAB cultures are activated and cultivated for fermentation and bioprocessing procedures.
09:31
Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres
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Cited by 2 •
2016
Here, we present methodology to generate and administer compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres to intact mouse islets in culture with subsequent immunofluorescence analysis of β-cell proliferation. This method is suitable for determining the efficacy of candidate β-cell mitogens.
13:46
A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
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Cited by 18 •
2016
In this work, poly(lactic acid)/polyethylene glycol (PLA/PEG) scaffolds were prepared by using a combination of melt mixing and selective leaching. The method herein discussed permitted to develop three-layer scaffolds by highly controlling both porosity and pore size. The mechanical properties were also evaluated in a physiological environment.
FAQs
In water, lactic acid undergoes partial dissociation rather than converting completely into ions. This process releases protons and forms lactate, establishing the chemical basis of its acidic behavior. Because the dissociation is partial, aqueous solutions contain both undissociated lactic acid and lactate. This distinction is important when describing acidity and the chemical forms present in solution.
The two enantiomers have the same molecular formula and connectivity but differ in their three-dimensional arrangement. That spatial difference can affect how each form interacts with biological systems, producing different biological activity. Lactic acid therefore provides a useful chemistry example of stereochemistry, showing that molecular shape, not only elemental composition, can influence biological behavior.
The molecule combines two different functional groups, giving it more than one chemically important feature. The carboxylic acid group is associated with proton release and lactate formation in water, while the hydroxyl group contributes to the molecule’s broader reactivity. Their combination helps explain why lactic acid is significant in functional-group chemistry as well as in practical formulations and materials.
Microorganisms generate lactic acid through fermentation of carbohydrates. In this process, carbohydrate starting materials are converted through microbial activity into the acid product. The same broader chemistry is connected to cellular metabolism, where pyruvate can be converted to lactate during anaerobic glycolysis. This makes lactic acid relevant to both biochemical pathways and microbial production processes.
In food applications, lactic acid helps regulate acidity and contributes to preservation. Adjusting acidity can influence the chemical environment of a product, while the preservation role supports maintaining food quality. Its use illustrates how an organic acid can provide both a functional formulation effect and a practical food-processing benefit without serving only as a flavor or chemical ingredient.
Lactic acid serves as a starting material for manufacturing biodegradable polylactic acid plastics and also appears in pharmaceutical and cosmetic formulations. These uses arise from the molecule’s structure and reactivity, which support both materials production and formulated products. Consequently, lactic acid connects fundamental chemistry with biodegradable materials, medicines, and personal-care applications.