Patient Isolation
Patient Isolation - Related Videos
11:10
Isolation of Human Islets from Partially Pancreatectomized Patients
0 Views •
Cited by 10 •
2011
The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.
07:32
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
0 Views •
Cited by 36 •
2012
Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture purities.
11:26
Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
0 Views •
Cited by 19 •
2015
This protocol describes techniques for live cell isolation and primary culture of myogenic and fibroblast cell lines from muscle or skin tissue. A technique for the immortalization of these cell lines is also described. Altogether, these protocols provide a reliable tool to generate and preserve patient-derived cells for downstream applications.
04:21
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
0 Views •
2023
The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.
12:16
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
0 Views •
Cited by 22 •
2013
This article describes the preparation of freshly obtained melanoma tissue into primary cell cultures, and how to remove contaminations of erythrocytes and fibroblasts from the tumor cells. Finally, we describe how CD133+ putative melanoma stem cells are sorted from the CD133- bulk using Magnetic Activated Cell Sorting (MACS).
FAQs
Patient isolation becomes more effective when precautions match the suspected route of transmission. Contact, droplet, and airborne routes require different combinations of controls because exposure occurs through different pathways. This approach helps clinicians select room placement, hand hygiene, personal protective equipment, and, when indicated, ventilation or respiratory protection without applying the same measures indiscriminately.
No single control provides the full protection needed in every clinical situation. Room placement limits proximity, hand hygiene reduces transfer, protective equipment adds a barrier, dedicated equipment reduces sharing, and environmental cleaning addresses contamination in the care space. Combining these measures creates overlapping safeguards that protect patients, healthcare workers, and visitors more reliably than any one measure alone.
Specialized ventilation and respiratory protection become relevant when airborne transmission is a concern. Ventilation helps limit the presence or movement of infectious particles in the clinical environment, while respiratory protection helps reduce exposure for people providing or receiving care. Their use adds a higher level of control beyond routine room placement, hygiene, equipment, and environmental measures.
The main difference is the transmission route that precautions are designed to interrupt. Contact precautions emphasize limiting transfer through people or shared surroundings, droplet precautions address spread through respiratory droplets, and airborne precautions may require specialized ventilation and respiratory protection. Matching controls to the route makes infection prevention more targeted while supporting necessary diagnosis and treatment.
A practical workflow begins when a transmissible infection is suspected or confirmed. Clinicians identify the likely route of spread, arrange appropriate room placement, perform hand hygiene, select personal protective equipment, and use dedicated equipment when appropriate. Environmental cleaning completes the control process, while specialized ventilation and respiratory protection are added when airborne exposure requires them.
The core resources include suitable room placement, hand-hygiene supplies, personal protective equipment, dedicated patient-care equipment, and environmental cleaning procedures. For situations involving airborne particles, specialized ventilation and respiratory protection may also be needed. Together, these resources reduce opportunities for transmission within healthcare environments while allowing clinicians to continue delivering timely care.
Appropriate isolation helps limit healthcare-associated transmission, which is especially important when several patients may be exposed during an outbreak. By reducing spread, it supports safer clinical care and protects healthcare workers and visitors. The overview also links correct isolation practices with antimicrobial stewardship, while maintaining timely diagnosis and treatment for the affected patient.