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Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
 

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

Article doi: 10.3791/52307
January 18th, 2015

Summary January 18th, 2015

This protocol describes techniques for live cell isolation and primary culture of myogenic and fibroblast cell lines from muscle or skin tissue. A technique for the immortalization of these cell lines is also described. Altogether, these protocols provide a reliable tool to generate and preserve patient-derived cells for downstream applications.

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