Cell Persistence Biodistribution
Cell Persistence Biodistribution - Related Videos
13:29
Biodistribution of Nano-drug Carriers: Applications of SEM
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Nanoparticles have been increasingly used research towards targeted drug delivery and controlled drug release. While most of these particles have been developed as polymeric or liposomal particles because of their biocompatibility, there is a trend in current research toward the use of metallic and magnetic nanoparticles. These metallic nanoparticles...
04:54
In Vitro Persister Assay: A Method to Evaluate the Effect of Osmolytes on Bacterial Persistence
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2025
In this video, we demonstrate a method to evaluate the effect of osmolytes on the formation of bacterial persisters. The bacterial cells are first exposed to osmolytes to induce osmotic stress, triggering persister formation, then treated with antibiotics to enumerate the persisters.
01:35
Assessing Cell Layer Permeability in Non-Replicating Persistent Mycobacterium tuberculosis
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2026
Begin with tubes containing the non-replicating persistent, or NRP stage of Mycobacterium tuberculosis, which has a thickened outer layer.Add glass beads to one tube and shake it to mechanically disrupt the mycobacteria’s outer layer, enhancing permeability. Next, transfer the bead-beaten mycobacterial suspension to a fresh tube.Add fluorescently-labeled rifampicin, an anti-tuberculosis antibiotic, to both the untreated and bead-beaten cell suspensions.Incubate with shaking to allow...
06:15
An In Vivo Immunofluorescence Localization Method to Study Antibody Biodistribution
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2025
This video demonstrates an in vivo immunofluorescence localization (IVIL) technique to study the biodistribution of tumor-targeting antibodies in a murine model of breast cancer. Upon the intravenous injection of a tumor-specific antibody in the mouse, the tumor is surgically isolated, and the localization of the antibody within the tumor tissue is confirmed via ex vivo immunostaining.
13:35
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
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Cited by 25 •
2017
This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [18F]-FDG) using PET/CT.
FAQs
Tissue homing directs introduced cells toward particular developmental sites, while engraftment reflects their retention and integration after arrival. Local microenvironmental signals can support continued survival, proliferation, or maintenance of cell identity. In contrast, apoptosis and immune-mediated clearance reduce persistence. Examining these interacting processes helps explain why cells accumulate in some tissues but disappear or remain sparse in others.
Persistence reflects the balance between processes that maintain a cell population and those that remove it. Proliferation can increase the number of retained cells, whereas apoptosis and immune-mediated clearance can decrease it. The surrounding microenvironment also influences survival and identity. Measuring these factors together provides a more informative view than treating cell disappearance as a single, unexplained outcome.
Finding cells at an intended site shows that they reached or remained in that tissue, but it does not by itself establish integration or continued identity. Developmental analysis therefore considers distribution alongside engraftment, lineage behavior, and identity measurements. This distinction helps separate transient localization from outcomes in which cells are retained and contribute meaningfully to tissue-level developmental patterns.
Patterns of persistence and tissue distribution provide evidence about where introduced cells travel, which sites retain them, and whether their identity is maintained. When combined with lineage-focused analyses, these observations can clarify cell fate and tissue integration. The resulting information helps investigators connect cell movement and survival with developmental organization rather than evaluating location as an isolated measurement.
A typical assessment begins by labeling the introduced cells, followed by imaging to examine their location and persistence. Tissue-based analyses then evaluate where cells are present and can help assess retention and identity. Using these approaches together allows researchers to compare observed distributions with intended developmental sites and to identify whether cells remain detectable across the analysis.
Cell labeling supports recognition of the introduced population, while imaging reveals its distribution and localization within the organism. Tissue-based analyses add information about retained cells and their identity in specific sites. Because each approach addresses a different aspect of the result, combining them strengthens interpretation of whether cells reached relevant developmental regions and remained associated with those tissues.
These measurements are useful when researchers need to determine whether introduced cells reach intended developmental sites, remain present, and maintain their identity. In cell-based models and regenerative strategies, the findings help evaluate performance and safety by revealing tissue distribution, retention, lineage behavior, and integration. They also identify patterns that may require closer examination when localization does not match the intended outcome.