Rx3:gfp Zebrafish
Rx3:gfp Zebrafish - Related Videos
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42 •
2011
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
08:07
Zebrafish Reproduction and Development
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2023
The zebrafish (Danio rerio) has become a popular model for studying genetics and developmental biology. The transparency of these animals at early developmental stages permits the direct visualization of tissue morphogenesis at the cellular level. Furthermore, zebrafish are amenable to genetic manipulation, allowing researchers to determine the effect of gene expression on the development of a vertebrate with a high degree of genetic similarity to humans. This video provides a brief overview of...
05:40
Tol2 Transposon-Mediated Zebrafish Transgenesis: A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos
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2025
In this video, we demonstrate a procedure to generate a stable zebrafish transgenic line constitutively expressing a fluorescent voltage indicator protein following the co-injection of the Tol2 transposon system comprising the transposase mRNA and Tol2 transposon construct encoding the fluorescent voltage indicator.
07:58
Zebrafish Maintenance and Husbandry
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2023
The zebrafish (Danio rerio) is a powerful vertebrate model system for studying development, modeling disease, and screening for novel therapeutics. Due to their small size, large numbers of zebrafish can be housed in the laboratory at low cost. Although zebrafish are relatively easy to maintain, special consideration must be given to both diet and water quality to in order to optimize fish health and reproductive success. This video will provide an overview of zebrafish husbandry and...
FAQs
Fluorescence arises when rx3 regulatory sequences activate GFP production in cells expressing the developmental program. The resulting signal marks rx3-expressing progenitor cells, so microscopy can reveal where those cells are located and how their positions change during development. This links gene activity with cellular behavior in the living embryo.
The reporter is valuable because the same embryo can be examined noninvasively as development proceeds. Fluorescence observations can follow changing cell locations and identities rather than relying only on a fixed developmental snapshot. In developmental biology, this temporal view helps connect early progenitor behavior with later formation of neural structures.
The fluorescence domain connects rx3 activity with two major developmental territories: the early eye field and hypothalamus. Observing these regions supports investigation of optic vesicle formation, retinal differentiation, and hypothalamic development. This makes the line useful for relating localized progenitor behavior to the coordinated neural patterning events that shape vertebrate organs.
To use Rx3:gfp Zebrafish for developmental observation, researchers examine living embryos with fluorescence microscopy and identify the GFP-marked progenitor population. They can then document its location, movements, and changing identity as development proceeds. This workflow provides a noninvasive way to study neural patterning, optic vesicle formation, retinal differentiation, or hypothalamic development.
Researchers can follow GFP-marked rx3-expressing progenitor cells as their positions and identities change over time. These observations provide a basis for connecting an early fluorescent population with developmental outcomes in the eye field or hypothalamus. Consequently, the model helps examine how progenitor behavior contributes to neural patterning and organ formation.
Genetic and chemical screens can use the fluorescent rx3 response as a way to identify perturbations associated with altered developmental patterns. Because the line highlights progenitor populations involved in the eye field and hypothalamus, screening results can be related to optic vesicle formation, retinal differentiation, hypothalamic development, or broader mechanisms of vertebrate organ formation.