Nano Biological Interfaces
Nano Biological Interfaces - Related Videos
10:28
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
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Cited by 5 •
2017
This paper presents a series of protocols for developing engineered cells and functionalized surfaces that enable synthetically engineered E. coli to control and manipulate programmable material surfaces.
11:56
Fluorescence Imaging with One-nanometer Accuracy (FIONA)
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Cited by 11 •
2014
Single fluorophores can be localized with nanometer precision using FIONA. Here a summary of the FIONA technique is reported, and how to carry out FIONA experiments is described.
10:25
Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
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Cited by 39 •
2016
We present a method for achieving sub-nanometer resolution images with amplitude-modulation (tapping mode) atomic force microscopy in liquid. The method is demonstrated on commercial atomic force microscopes. We explain the rationale behind our choices of parameters and suggest strategies for resolution optimization.
02:04
Protein-protein Interfaces
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2020
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
11:27
Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
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Cited by 33 •
2016
Here, we present a protocol to investigate soft matter and biophysical systems over a wide mesoscopic length scale, from nm to µm that involves the use of the KWS-2 SANS diffractometer at high intensities and an adjustable resolution.
FAQs
Surface chemistry helps determine which proteins adsorb at the interface and how that adsorption occurs. Because adsorbed proteins form an immediate molecular layer between the engineered material and its biological surroundings, their presence can influence later cellular attachment, communication, and response. Controlling surface chemistry therefore helps engineers connect material performance with biological specificity and improve interface behavior.
Nanoscale topography and surface charge are key variables that shape how cells interact with an engineered boundary. Along with molecular recognition, they can affect cell attachment, communication, and broader cellular responses. Adjusting these properties gives engineers ways to tune an interface for a particular biological function rather than relying only on the material’s bulk characteristics.
Molecular recognition provides biological specificity at the interface by helping the engineered system interact with selected biomolecules or cellular features. This selectivity is important when an interface must detect a biological signal or support targeted therapeutic delivery. Combined with surface chemistry, topography, and charge, recognition helps determine whether the system produces a useful response or an unwanted one.
Design requires coordinating interfacial properties with the intended engineering function. Surface chemistry, nanoscale structure, charge, and recognition must support the desired biological interaction while preserving useful material behavior. This balance is especially important for systems that contact tissues or cells, because improved integration and performance must be pursued while reducing unwanted immune or inflammatory responses.
These interfaces support several engineering applications, including biosensors, drug-delivery systems, tissue scaffolds, and implantable devices. In biosensors, interfacial recognition can contribute to signal detection. In delivery systems, biological specificity can support therapeutic targeting. Scaffolds and implants rely on controlled interactions with cells or tissues to improve device integration and biological compatibility.
Evaluation can focus on how effectively the interface supports signal detection, therapeutic targeting, tissue or device integration, and biocompatibility. Researchers also examine cellular attachment, communication, and response, together with unwanted immune or inflammatory effects. These outcomes connect nanoscale design choices to practical engineering performance and help identify which interfacial properties require further adjustment.