High Throughput

High-throughput methods are approaches that process and analyze many samples, measurements, or experimental conditions in parallel, enabling rapid generation of large datasets. They typically combine automated handling, miniaturized assays, multiplexed detection, and computational analysis to increase speed, consistency, and experimental scale. In environmental sciences, high-throughput workflows can screen pollutants, characterize microbial communities, assess soil or water quality, and compare ecological responses across locations or conditions. These approaches help researchers identify patterns, prioritize samples for detailed study, and monitor environmental change more efficiently, while also creating data resources for risk assessment, ecosystem management, and predictive modeling.

High Throughput - Related Videos

Research

JoVE Journal - Biology

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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2015

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

Research

JoVE Journal - Biochemistry
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High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

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Cited by 16 •

2023

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

Research

JoVE Journal - Medicine
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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

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Cited by 3 •

2020

We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1.

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

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Cited by 117 •

2016

In this manuscript, we present a high-throughput, semi-automated cryohistology platform to produce aligned composite images of multiple response measures from several rounds of fluorescent imaging on frozen sections of mineralized tissues.

Research

JoVE Journal - Biology
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High-throughput Titration of Luciferase-expressing Recombinant Viruses

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Cited by 14 •

2014

This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.

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