Photosynthetic Rates

Photosynthetic rates quantify how quickly photosynthetic organisms convert light energy into chemical energy, commonly measured through carbon dioxide uptake or oxygen production. In chloroplasts, light-driven electron transport generates ATP and NADPH, which power carbon fixation in the Calvin cycle; the rate changes with light intensity, carbon dioxide availability, temperature, and water status. In genetics, comparing photosynthetic rates among plant varieties or engineered lines helps reveal how genes influence chloroplast function, enzyme activity, and stress responses. These measurements support research on plant productivity, crop improvement, adaptation to environmental change, and the genetic basis of complex traits.

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JoVE EoE - Bacterial Growth and Techniques

Extraction and Purification of Plastoglobules from Photosynthetic Bacteria

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2025

Source: Shivaiah, K., et al. Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria. J. Vis. Exp. (2022)The video demonstrates the isolation of plastoglobules from cyanobacteria. It begins with a culture of cyanobacteria and proceeds through cell disruption using mechanical force. The release of photosynthetic pigments indicates successful lysis. A sucrose step gradient is then used to separate cellular components by density, allowing low-density plastoglobules to form a...

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JoVE Journal - Biology
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High-Throughput, In-Field Screening of Photosynthetic Efficiency in Crop Plants Using an Autonomous Robot

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2026

This manuscript describes a non-destructive, high-throughput approach for autonomous field measurements of photosystem II quantum efficiency, spectral reflectance, and plant architecture, enabling large-scale canopy photosynthesis phenotyping in agronomic and breeding field trials.

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

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2025

Here, we describe methods of sampling from the euphotic zones of marine and freshwater ecosystems to isolate cyanophages (and their hosts) and post-sampling processes that enrich single genotypes and characterize virus-host interaction dynamics.

Construction and Setup of a Bench-scale Algal Photosynthetic Bioreactor with Temperature, Light, and pH Monitoring for Kinetic Growth Tests

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Cited by 4 •

2017

This paper describes the assembly process and operation of a bench-scale photosynthetic bioreactor that can be used, in conjunction with other methods, to estimate pertinent kinetic growth parameters. This system continuously monitors the pH, light, and temperature using sensors, a data acquisition and control unit, and open-source data acquisition software.

Comparison of Scale in a Photosynthetic Reactor System for Algal Remediation of Wastewater

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Cited by 3 •

2017

An experimental methodology is presented to compare the performance of small (100 L) and large (1,000 L) scale reactors designed for algae remediation of landfill wastewater. System characteristics, including surface area to volume ratio, retention time, biomass density, and wastewater feed concentrations, can be adjusted based on application.

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