Oncogene Discovery
Oncogene Discovery - Related Videos
03:07
Immortalizing Endothelial Cells via Retroviral Transduction of Viral Oncogenes
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2026
Source: McAllister, S. C., Hanson, R. S., et al. An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus. J. Vis. Exp.(2017)This video demonstrates retroviral transduction of primary endothelial cells with HPV16 E6/E7 oncogenes. The integrated oncogenes inactivate p53 and Rb tumor suppressors, extending cell lifespan. G418 selection enriches for transduced cells, yielding immortalized endothelial cultures suitable for transformation...
13:37
Polymer Microarrays for High Throughput Discovery of Biomaterials
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Cited by 32 •
2012
A description of the formation of a polymer microarray using an on-chip photopolymerization technique. The high throughput surface characterization using atomic force microscopy, water contact angle measurements, X-ray photoelectron spectroscopy and time of flight secondary ion mass spectrometry and a cell attachment assay is also described.
08:18
Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
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Cited by 23 •
2014
The Focus Formation Assay provides a straightforward method to assess the transforming potential of a candidate oncogene.
01:33
Cancer-Critical Genes I: Proto-oncogenes
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2023
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype. When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors. Such genes that act...
01:26
Drug Discovery: Overview
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2023
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
FAQs
These categories describe different ways a proto-oncogene can become cancer-promoting. Activation can increase gene activity, amplification can raise its copy number, and altered regulation can disrupt normal control. Separating them helps researchers connect a specific genetic change with effects on proliferation, survival, or differentiation in tumors.
Gene fusions and copy-number changes provide complementary clues during oncogene discovery. Alongside mutations, they help researchers identify genetic alterations associated with tumor behavior and disease progression. Examining these alteration types broadens the search beyond mutations alone and supports a more complete genetic picture of how particular tumors acquire growth-promoting abnormalities.
Functional assays are important because a detected alteration does not by itself establish how it affects cancer biology. Researchers use these assays to test whether a candidate change can influence uncontrolled growth-related processes, including proliferation, survival, or differentiation. This evidence helps distinguish a biologically meaningful driver from a genetic observation associated with a tumor.
A typical discovery workflow compares tumor and normal genomes, analyzes gene expression, and then applies functional assays to candidate changes. This combination connects genomic differences with altered activity and tests their relevance to proliferation, survival, or differentiation. The resulting evidence can prioritize alterations for further genetic and cancer research.
Once linked oncogenes are established, they can support cancer classification by distinguishing tumor groups with different genetic features. They also contribute to biomarker development and risk assessment, while providing molecular targets for designing targeted therapies. These uses translate discovery findings into tools for cancer research and therapeutic development.
Continued genomic studies matter because tumor genetics can reveal not only mechanisms of tumor initiation but also changes associated with treatment resistance. By extending analysis beyond the first growth-promoting alteration, researchers can refine links between genetic events and disease progression. This ongoing work may expose additional oncogenic mechanisms relevant to cancer classification and therapeutic research.