Placental Crispr Manipulation
Placental Crispr Manipulation - Related Videos
07:39
Mouse In Vivo Placental Targeted CRISPR Manipulation
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Cited by 5 •
2023
Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.
02:53
CRISPR and crRNAs
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2020
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea. The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
05:22
Ex vivo Culture of Human Placental Explants for the Study of Viral Transmission Across the Maternal-Fetal Interface
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2025
This protocol describes the preparation of human placental tissue explants cultured at an air-liquid interface for the study of viral infections.
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 49 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
12:17
The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo
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Cited by 19 •
2017
We present a detailed method to study human placental physiology in vivo at term. The method combines blood sampling from the incoming and outgoing vessels on the maternal and fetal sides of the placenta with ultrasound measurements of volume blood flow and placental tissue sampling.
FAQs
Specificity comes from the guide RNA’s ability to direct Cas9 to a selected DNA sequence. Cas9 then creates a break at that site, making the experiment’s central question gene-specific rather than a general change to placental DNA. This targeted design allows investigators to connect altered gene activity with particular placental functions or disorders.
The cellular repair route determines what the edit means. Repair can disrupt the target gene, replace a sequence, or regulate its activity, so the same CRISPR framework can support different experimental questions. Comparing these outcomes helps researchers distinguish whether a placental function depends on loss of a gene, an altered sequence, or changed regulation at the target.
These systems provide different settings for examining gene function in placental biology. Trophoblast cultures and placental organoids support studies in placental cell-based systems, while experimental models extend investigation beyond those systems. Using these platforms helps researchers relate a targeted genetic change to processes such as placental formation, transport, hormone production, or disorder-related mechanisms.
A study begins by selecting a DNA sequence associated with the placental gene or process of interest. Researchers then use a guide RNA to direct a CRISPR-associated nuclease, such as Cas9, to that sequence. After the nuclease creates a DNA break, the resulting repair outcome is examined in a trophoblast culture, organoid, or experimental model.
The approach can link individual genes to implantation, nutrient and oxygen transport, hormone production, and placental development. It can also help identify how genetic changes contribute to placental disorders. These outcomes connect a targeted DNA alteration with a specific function, giving genetics researchers a way to investigate mechanisms at the maternal-fetal interface.
It is useful when researchers need to test whether a particular gene contributes to placental function or disease mechanisms. By applying targeted changes in placental cells or experimental models, investigators can examine gene roles in the maternal-fetal interface and evaluate potential interventions. The method therefore connects molecular genetics with placental biology and translational disease research.