Annexin V Red
Annexin V Red - Related Videos
09:09
Annexin V and Propidium Iodide Labeling
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2023
Staining with annexin V and propidium iodide (PI) provides researchers with a way to identify different types of cell death—either necrosis or apoptosis. This technique relies on two components. The first, annexin V, is a protein that binds certain phospholipids called phosphatidylserines, which normally occur only in the inner, cytoplasm-facing leaflet of a cell’s membrane, but become “flipped” to the outer leaflet during the early stages of apoptosis. The second component is the DNA-binding...
04:21
Annexin V Binding Assay: A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling
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2025
In this video, we demonstrate the Annexin V binding assay to measure the degree of eryptosis, erythrocyte programmed cell death, using fluorescent Annexin V staining. Apoptotic erythrocytes demonstrate phosphatidylserine translocation from the inner to the outer leaflet of the cell membrane, causing the fluorophore-tagged Annexin V to bind to the translocated phosphatidylserine, thereby emitting a fluorescent signal.
07:34
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
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Cited by 543 •
2011
An accurate method for the assessment of cell death is described. The protocol improves upon conventional Annexin V/ propidium iodide (PI) protocols, which display up to 40% false- positive events in cell lines and primary cells from a broad range of animal models.
01:23
Red Algae
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2025
Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
09:12
Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
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Cited by 51 •
2018
Here we present techniques to measure red cell deformability and cellular heterogeneity by ektacytometry. These techniques are applicable to general investigations of red cell deformability and specific investigations of blood diseases characterized by the presence of both rigid and deformable red cells in circulation, such as sickle cell anemia.
FAQs
Calcium ions enable the annexin V component to bind phosphatidylserine exposed on the cell surface. Without this calcium-dependent interaction, externalized phosphatidylserine would not be efficiently recognized by the reagent, reducing the fluorescent signal. Maintaining the appropriate calcium-containing conditions is therefore important when assessing apoptotic or cell-death-associated membrane changes.
Phosphatidylserine normally resides on the inner leaflet of the plasma membrane, but apoptosis is associated with its movement to the outer leaflet. Annexin V Red detects this altered distribution, allowing researchers to identify an early membrane change linked to apoptosis. This makes the signal useful for following cell responses before later stages of cell death are assessed.
Its red fluorophore converts annexin V binding into a fluorescence signal that can be measured by different platforms. Fluorescence microscopy provides visual information about labeled cells, whereas flow cytometry measures fluorescence across a cell population. Choosing between them depends on whether the experiment emphasizes cellular imaging or population-level analysis of immune or infected cells.
Cells are exposed to the experimental condition, evaluated with Annexin V Red under calcium-containing conditions, and then examined by fluorescence microscopy or flow cytometry. A membrane-impermeable dye such as propidium iodide can be included when researchers need to separate viable, apoptotic, and necrotic populations. The resulting fluorescence patterns support comparison between treatment or infection conditions.
Annexin V Red reports phosphatidylserine exposure, while propidium iodide adds a membrane-impermeability readout. Considering both signals provides more information than either measurement alone and helps distinguish viable, apoptotic, and necrotic cells. This combined approach is particularly useful when an experiment must determine whether a pathogen or antimicrobial treatment is associated with different stages of cell injury.
The reagent can help examine pathogen-induced cellular injury, immune-cell responses, and the effects of antimicrobial treatments. Researchers may compare fluorescence patterns between untreated and experimentally challenged cells to determine whether conditions are associated with phosphatidylserine exposure or broader cell death. These measurements provide a way to connect infection or treatment conditions with changes in cell viability.