Human Tonsil Processing
Human Tonsil Processing - Related Videos
08:09
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
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Cited by 11 •
2015
Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.
01:30
Tonsillitis I: Introduction
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2024
Tonsillitis is inflammation of the tonsils, which are two lymphoid tissue masses at the back of the throat. This condition can cause discomfort and irritation in the throat. Etiology Three primary contributing factors have been identified. • Viruses: Viral infections are the most common cause of tonsillitis. Common viruses responsible for tonsillitis include rhinovirus, influenza, parainfluenza, Epstein-Barr virus, and adenovirus. • Bacteria: The predominant bacterium responsible for...
01:26
Tonsillitis II: Management
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2024
This lesson will focus on the different treatment options for managing tonsillitis, which typically depend on the cause and severity. • Acute viral tonsillitis treatment usually involves outpatient supportive management, including analgesia (acetaminophen) and oral hydration, corticosteroids (dexamethasone), salt-water gargles, and rest. • Bacterial tonsillitis is typically treated with penicillin as the primary choice, although cephalosporins can also be effective. Antibiotic therapy with...
13:08
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture
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Cited by 80 •
2013
A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.
11:36
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
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Cited by 2 •
2016
The RAPID blood processing method can be used in humans and yields higher peptide levels as well as allows for assessment of the correct molecular form. Therefore, this method will be a valuable tool in peptide research.
FAQs
Sterile handling helps preserve the interpretability of tonsil samples by limiting contamination during preparation. This matters when investigators culture cells or examine microbial material, because introduced contaminants could be confused with infection-associated findings or alter cell behavior. Maintaining controlled handling therefore supports more reliable analysis of local immune responses and improves comparisons among samples processed for immunology and infection studies.
Mechanical dissociation physically separates the tissue, while enzymatic digestion can further help release cells from the tissue structure. Using either approach, or combining them when appropriate, allows investigators to obtain material suited to downstream analysis. The choice influences whether the preparation is a viable single-cell suspension or a set of defined tissue fractions, which affects the type of measurements possible.
Consistent handling is central to meaningful comparisons. Similar preparation of surgically collected samples, including controlled tissue handling, dissociation, filtration, and centrifugation, helps ensure that observed differences reflect biology rather than variable sample preparation. This standardization is especially important when evaluating immune-cell activation, local infection responses, or differences in the cellular composition of healthy and diseased tonsils.
After collection, the tissue is handled under sterile conditions and trimmed before dissociation. The resulting material may undergo enzymatic digestion, followed by filtration and centrifugation. These steps produce either a viable single-cell suspension or defined tissue fractions. The prepared material can then be directed to flow cytometry, cell culture, molecular profiling, or pathogen-focused investigation, depending on the study goal.
Processing can provide access to lymphocytes, antigen-presenting cells, epithelial cells, and microbial material. This range is valuable because tonsils contain both immune and tissue-associated components relevant to mucosal immunity and infection. Separating or preserving these materials in suitable fractions allows investigators to connect cellular composition with immune-cell activation, local infection responses, and host-pathogen interactions.
It supports studies that examine immune responses within a local tonsil tissue environment. Researchers can use prepared samples for flow cytometry, cell culture, molecular profiling, and pathogen research while comparing healthy and diseased tissue. These applications help investigate mucosal immunity, immune-cell activation, local infection responses, and interactions between host tissue and microbial material.