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JoVE Journal
Medicine

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Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture
 

Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture

Article DOI: 10.3791/50011
January 3rd, 2013

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Summary January 3rd, 2013

A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.

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