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Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture

Article DOI: 10.3791/50011-v • 13:08 min • January 3rd, 2013
January 3rd, 2013 •
1, 1, 1
1Life Science Division, Lawrence Berkeley National Laboratory

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Summary
Automatic Translation

A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.

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Human Reduction Mammoplasty Mastectomy Tissues Cell Culture Mammary Epithelial Cells In Vitro Culture Systems Human Derived Material Cellular Differentiation Aging Senescence Immortalization Mammary Gland Epithelial Cells Adipose Cells Mesenchymal Cells Endothelial Cells Breast Cancers Surgical Discard Tissues Primary Culture Digestion
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