Lung Tissue Biobanking
Lung Tissue Biobanking - Related Videos
04:37
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
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2023
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
08:42
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
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Cited by 25 •
2023
Presented here is a reproducible, affordable, and robust method for the isolation and expansion of primary bronchial epithelial cells for long-term biobanking and the generation of differentiated epithelial cells by culture at the air-liquid interface.
06:42
Optimization of Breast Biopsy and Mastectomy Sample Collection Procedures for Biobanking, Personalized Medicine, and Research Applications
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2025
This article presents a detailed protocol for collecting breast cancer samples, designed to optimize their utility for biobanking, personalized medicine, and other research applications. The workflow for sample collection, including breast biopsy and mastectomy procedures, is described in detail, focusing on minimizing tissue damage and maximizing sample quality.
03:03
Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs
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2025
In this video, we describe a surgical procedure to extract bronchiolar tissues from harvested pig lung. The ex vivo bronchiolar tissue could be used to develop infection models for studying chronic lung infections.
03:48
Mouse Lung Preparation for Flow Cytometry: Generating Cell Suspension from Lung Tissue for Flow Cytometric Analysis
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2023
This video describes the preparation of mouse lung cell suspension for flow cytometric analysis to study PD-L1 expression of lung adenocarcinoma cells.
FAQs
Dividing a specimen preserves different research capabilities within the same case. Fixed tissue supports examination of morphology, while cryopreserved material helps retain biological molecules for later analysis. Separately isolating viable cells maintains cellular function for experiments that require living material. This coordinated allocation broadens the questions researchers can address without relying on additional tissue.
Clinical metadata connects each specimen to relevant information about its source and context. That linkage allows researchers to interpret tissue morphology, nucleic acids, proteins, or cellular function alongside clinical characteristics rather than as isolated measurements. Consistent annotation also supports comparisons among specimens, diseases, and time points, strengthening interpretation across studies.
Standardized handling reduces variation introduced during collection, processing, and storage. Quality control helps determine whether preserved material remains suitable for the intended analysis and makes results more comparable between specimens or studies. Together, these practices improve reproducibility, support reliable interpretation of biological findings, and reduce dependence on obtaining newly collected samples.
Repositories provide access to lung material for examining host responses, pathogen distribution, and tissue injury within affected organs. Researchers can relate these findings to disease and treatment context, helping distinguish changes in immune activity from broader structural damage. The stored specimens also support investigations across different time points when comparable material is available.
Following surgical resection, biopsy, or autopsy, the tissue is handled under controlled conditions and divided according to planned research needs. Portions may undergo fixation, cryopreservation, or isolation of viable cells, while associated clinical information is recorded. The processed material is then stored and made available for appropriately matched future studies.
The choice depends on the biological information the study needs to preserve. Fixed portions are appropriate when tissue morphology is central, whereas cryopreserved samples retain material for later analysis of biological molecules. Viable-cell isolation is selected when cellular function matters. Using more than one preparation can support complementary analyses from a single specimen.
Annotated collections can support comparisons of host responses, pathogen distribution, tissue injury, and treatment effects across diseases or time points. Because specimens are processed and documented systematically, researchers can examine patterns across cases rather than relying only on individual samples. This comparative capacity is especially useful when newly collected tissue is limited or unavailable.