Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Isolating Bronchial Epithelial Cells from…
JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
Journal (Immunology and Infection)
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
Journal (Immunology and Infection)
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
 

Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

Article DOI: 10.3791/65102-v • 08:42 min • May 26th, 2023
May 26th, 2023 •
1, 1, 1
1PulmoScience Laboratory, Department of Pulmonology, Leiden University Medical Center

Chapters

Summary
Automatic Translation

Presented here is a reproducible, affordable, and robust method for the isolation and expansion of primary bronchial epithelial cells for long-term biobanking and the generation of differentiated epithelial cells by culture at the air-liquid interface.

Tags

Isolating Bronchial Epithelial Cells Resected Lung Tissue Biobanking Well-differentiated Air-liquid Interface Cultures Research Questions Function Of Airway Epithelial Cells Health And Disease Advantages Of The Technique Cell Layer Representation Mucus Production Ciliary Activity Disease-related Insults Therapies Rinsing Bronchial Ring Connective Tissue Removal Blood Remnants Removal Incubation With Protease 14 Solution Scraping Inside The Ring Obtaining Cell Solution Centrifugation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter