Ovine Skin Model
Ovine Skin Model - Related Videos
10:32
Transvaginal Mesh Insertion in the Ovine Model
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Cited by 11 •
2017
This protocol describes mesh implantation in the ovine rectovaginal septum using a single vaginal incision technique, with and without the trocar-guided insertion of anchoring arms.
02:13
Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen
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2025
The video demonstrates the generation of an ex vivo ovine skin model infected with Staphylococcus aureus. This entails treating a lamb's forehead skin biopsy section with antibiotics, establishing a wound bed, positioning it on a permeable membrane in a multiwell plate with antibiotic-free medium, and establishing bacterial infection in the skin wound.
08:30
A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics
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2022
The protocol describes a step-by-step method to set up an ex vivo ovine wounded skin model infected with Staphylococcus aureus. This high-throughput model better simulates infections in vivo compared with conventional microbiology techniques and presents researchers with a physiologically relevant platform to test the efficacy of emerging antimicrobials.
07:25
Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury
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Cited by 18 •
2017
Intervertebral disc degeneration is a significant contributor to back pain and a leading cause of disability worldwide. Numerous animal models of intervertebral disc degeneration exist. We demonstrate an ovine model of intervertebral disc degeneration, utilizing a drill bit, which achieves a consistent disc injury and reproducible level of disc degeneration.
06:11
Anterior Cervical Discectomy and Fusion in the Ovine Model
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Cited by 9 •
2009
This video demonstrates the technique of anterior cervical discectomy and fusion in the ovine model.
FAQs
Its value comes from examining the epidermal barrier together with innate defenses, inflammatory cells, and adaptive immunity in the same skin system. This allows investigators to relate barrier disruption or tissue changes to cellular recruitment and cytokine production. The resulting measurements provide a tissue-level view of host responses that complements findings from laboratory studies.
Researchers can assess several response levels in parallel, including visible or measured tissue changes, recruitment of immune cells, cytokine production, and pathogen burden. Comparing these outcomes helps show whether a pathogen, antigen, or treatment primarily affects the local skin environment or also alters coordinated immune activity. This multidimensional approach strengthens interpretation of disease mechanisms.
A physiologically relevant skin setting can connect experimental exposure with tissue-level responses rather than relying only on isolated laboratory observations. In the Ovine Skin Model, investigators can examine how the barrier, innate defenses, inflammatory cells, and adaptive immunity respond together. That context helps clarify how infection develops and how treatment effects appear within skin.
The experimental design introduces or examines a pathogen, antigen, or treatment in sheep skin or living sheep under controlled conditions. Investigators then evaluate tissue changes, immune-cell recruitment, cytokine production, and pathogen burden. Relating these measurements to the experimental exposure helps determine how the intervention or infectious challenge affects skin biology and immunity.
Useful outcomes include changes in tissue appearance or structure, the recruitment of inflammatory and other immune cells, cytokine production, and the amount of pathogen detected. Together, these results can indicate whether an immune response has developed, how strongly the tissue is affected, and whether a treatment changes infection-related or inflammatory outcomes.
It is particularly useful when researchers need to connect immune mechanisms with changes occurring in skin tissue. Studies can investigate host responses to infectious disease, examine reactions to antigens, or evaluate treatment effects under controlled conditions. By linking laboratory findings with tissue responses, the model supports interpretation of both disease mechanisms and therapeutic outcomes.