In Vivo Confocal Microscopy
In Vivo Confocal Microscopy - Related Videos
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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Cited by 11 •
2014
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.
08:13
In Vivo Confocal Microscopy in the Diagnosis and Management of Dry Eye: A Focus on Imaging Protocols and Interpretation
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2025
This article demonstrates the operation and effect of in vivo confocal microscopy in the diagnosis and treatment of dry eye and investigates the application and outcomes of in vivo confocal microscopy to provide a basis for future research.
10:48
Imaging Biological Samples with Optical and Confocal Microscopy
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
09:16
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
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Cited by 18 •
2014
Time-lapse confocal imaging is a powerful technique useful for characterizing embryonic development. Here, we describe the methodology and characterize craniofacial morphogenesis in wild-type, as well as pdgfra, smad5, and smo mutant embryos.
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
FAQs
The imaging signal is sharpened by confocal optical sectioning. As the focused laser scans tissue, the confocal pinhole excludes light from regions outside the focal plane. This preserves information from a thin depth rather than mixing signals through the tissue. Repeated sections can then be combined, allowing cellular architecture to be examined with greater spatial detail in living tissue.
The confocal pinhole is important because it rejects out-of-focus light before the image is formed. That selectivity helps separate the focal layer from surrounding tissue, producing a clearer optical section than an image that combines light from multiple depths. Its role is therefore central to obtaining cell-level detail and distinguishing structures within thin tissue planes.
Compared with conventional histology, the method can examine tissue in its living state and does not require removal of a biopsy for each observation. That distinction supports real-time assessment and monitoring over the course of disease or treatment, while histology remains a conventional approach for evaluating removed tissue. The reduced reliance on biopsy-based evaluation can make longitudinal investigation more practical.
The main advantage of observing living tissue is access to changing biological information. Clinicians and researchers can evaluate cellular changes, follow disease progression, and assess responses to treatment. This extends investigation beyond a single fixed tissue snapshot and supports studies that depend on observing tissue states over time rather than examining only a removed specimen.
The cornea and skin are central use cases because they are accessible for optical examination. The same principle can support imaging of other accessible tissues, provided the tissue can be reached by the focused scan. These applications let investigators characterize cellular changes in clinically relevant locations and connect microscopic observations with disease assessment or treatment monitoring.
A typical acquisition directs a focused laser onto an accessible tissue while the system scans across the area of interest. The confocal pinhole blocks out-of-focus light, producing thin optical sections at selected focal regions. Those sections can then be combined into detailed images, enabling examination of cellular features without removing the tissue for the imaging session.
In medicine and biomedical research, the technique supports characterization of cellular changes, monitoring of disease progression, and assessment of treatment responses. It can also guide targeted investigations by showing tissue features at cell-level resolution in real time. These uses are especially relevant for the cornea, skin, and other tissues that can be accessed optically.