Scleral Fibroblasts
Scleral Fibroblasts - Related Videos
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
05:56
Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
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Cited by 27 •
2016
We demonstrate the effect of scleral crosslinking with riboflavin and UVA on an axial elongation rabbit eye. Axial elongation was induced in 13 day-old New Zealand rabbits (male and female) by suturing their right eye eyelids (tarsorrhaphy).
05:54
Rapid Culturing of Primary Murine Melanocytes and Fibroblasts: Isolating Melanocytes and Fibroblasts from Whole Murine Skin Sample
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2023
This video describes the rapid method to isolate and culture the melanocytes and fibroblast simultaneously from the postnatal mouse pup’s skin. These cells can be maintained and manipulated in vitro to study skin cell biology, wound healing, and melanoma.
06:04
Murine Dermal Fibroblast Isolation by FACS
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Cited by 28 •
2016
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
09:38
Direct Reprogramming of Mouse Fibroblasts into Melanocytes
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Cited by 2 •
2021
Here, we describe an optimized direct reprogramming system for melanocytes and a high-efficiency, concentrated virus packaging system that ensures smooth direct reprogramming.
FAQs
Mechanical loading, growth signals, and changes in the ocular environment can alter how scleral fibroblasts regulate extracellular-matrix production and remodeling. These inputs matter because the matrix determines much of the sclera’s structural organization. Studying their effects helps explain how the eye wall adapts during growth or changing conditions, with implications for eye size and biomechanical stability.
Collagen and proteoglycans are key extracellular-matrix components managed by scleral fibroblasts. Their synthesis and remodeling influence the organization and mechanical properties of the sclera rather than simply adding material. Changes in this balance can therefore affect how well the eye wall preserves its shape and resists structural alteration, making matrix regulation central to studies of ocular biomechanics.
Altered activity may change the extracellular matrix and, consequently, the structural behavior of the sclera. Because scleral structure influences eye size, abnormal remodeling is relevant to research on myopia, where investigators examine relationships among fibroblast behavior, scleral growth, and ocular biomechanics. This connection identifies fibroblast-controlled matrix regulation as a possible point for understanding disease mechanisms.
They can investigate how scleral growth is regulated, how extracellular-matrix remodeling affects eye-wall strength and shape, and why fibroblast activity changes in ocular disorders. Such studies connect cellular behavior with tissue-level biomechanics. The resulting information can support disease-mechanism research and help identify targets for approaches intended to regulate eye-wall structure.
Relevant outcomes include changes in collagen and proteoglycan synthesis or remodeling, alterations in scleral structure, and effects on eye size or biomechanical stability. Examining these levels together links cell activity to tissue behavior and ocular consequences. This integrated view helps distinguish a molecular change from a change that may influence the shape or mechanical integrity of the eye wall.
Their importance comes from the connection between extracellular-matrix regulation and clinically relevant ocular remodeling. Investigating these cells may clarify mechanisms associated with myopia and other disorders involving the eye wall, while also informing therapeutic-target research. The broader medical value lies in linking cellular processes to strategies that could regulate scleral biomechanics and preserve ocular structure.