A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Surgery, John A. Burns School of Medicine,University of Hawai’i
Chapters
- 00:05Title
- 00:53Murine Dermis Digestion
- 03:58Fibroblast FACS Isolation
- 04:46Results: Representative Microarray Analysis
- 05:36Conclusion
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
