Spheroid Culture Generation
Spheroid Culture Generation - Related Videos
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Spheroid Generation from Cell Lines: A Three-Dimensional (3D) Cell Culture Method
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2023
Spheroids are a type of 3D cell culture model used to more accurately represent a cell’s environment compared to 2D cell culture monolayer models. In the example protocol, we will see scientists generate spheroids from a colorectal cancer cell line and apply live-cell imaging to track the spheroids’ growth.
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Three-Dimensional Culture Systems: Spheroids and Organoids
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2026
Three-dimensional (3D) culture systems, including spheroids and organoids, support New Approach Methodology (NAM)-aligned research by providing more physiologically relevant models than conventional two-dimensional cell cultures. Because they more closely resemble the structure and cellular interactions of tissues and organs, these systems can provide physiologically relevant data before advancing to animal studies. Spheroids as Disease and Drug Screening Models Spheroids are generated when one...
03:18
Generation of a Three-Dimensional Spheroid Model of Tumor Cell Invasion
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2025
Source: Guyon, J., et al. A 3D Spheroid Model for Glioblastoma. J. Vis. Exp. (2020)The video demonstrates the generation of a three-dimensional spheroid model of tumor cell invasion. Spheroids are formed from human brain tumor cells and embedded in a collagen matrix. The tumor cells at the spheroid periphery adopt an invasive phenotype, secreting proteases to degrade the collagen and advancing to invade the surrounding matrix.
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Generation of Spheroids from Human Pluripotent Stem Cells
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2025
This video demonstrates the transformation of human pluripotent stem cells (hPSCs) into spherical brain organoids. By using specific inhibitors, the hPSCs are guided to differentiate towards a neuronal lineage, forming neuroectodermal colonies. Special growth factors are then introduced, which encourage the self-organization and proliferation of the colonies, ultimately leading to the formation of spherical brain organoids.
03:20
Splitting Spheroids for Sub-culturing and Shipping: A Procedure for Propagating and Transferring Spheroids from Small Bowel Neuroendocrine Tumor
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2023
This video demonstrates a strategy to split and propagate spheroids developed from small bowel neuroendocrine tumor cells. These spheroids have small bowel neuroendocrine tumor markers and can be propagated and further used to test anti-cancer drugs.
FAQs
Cell-cell adhesion drives dispersed cells to associate into compact clusters, while nonadherent or low-attachment conditions reduce attachment to the culture surface. Researchers can support aggregation through suspension, hanging-drop, or specialized microwell systems. The selected environment therefore helps determine whether cells remain organized as a three-dimensional cluster rather than spreading across a conventional culture surface.
As spheroids become compact, oxygen and nutrients may not be distributed uniformly throughout the cluster, while waste can accumulate along internal paths. These gradients create differences between cells located near the outside and those deeper within the structure. Such spatial variation makes spheroids useful for examining tissue-like responses that may be missed in more uniform culture conditions.
Spheroid culture preserves a three-dimensional arrangement that allows cellular interactions to occur within a compact structure. Conventional two-dimensional culture places cells on a flat surface and may not reproduce the same organization or internal gradients. Consequently, spheroids can provide a complementary model for evaluating biological responses before findings are considered in more complex systems.
Three supported formats are suspension culture, hanging drops, and specialized microwell systems. Each provides a setting in which dispersed cells can remain together under nonadherent or low-attachment conditions, allowing cell-cell adhesion to promote cluster formation. Choosing among these formats gives researchers a way to adapt spheroid generation to the experimental question and desired three-dimensional model.
A basic workflow begins with dispersed cells, places them in a nonadherent or low-attachment environment, and maintains conditions that support cell-cell adhesion and aggregation. Suspension, hanging-drop, or specialized microwell systems can provide that environment. The resulting compact clusters can then be used to study cellular behavior, treatment effects, or tissue responses in three dimensions.
Spheroids support disease modeling, cancer research, and drug screening because their three-dimensional organization can represent cellular interactions and internal gradients. They also help investigators study tissue responses and evaluate treatment effects in a model that differs from flat culture. These uses make spheroid systems relevant when researchers want laboratory findings to better inform studies of complex biological systems.