Spleen-preserving Pancreatectomy
Spleen-preserving Pancreatectomy - Related Videos
07:33
Robotic Spleen-Preserving Distal Pancreatectomy: The Warshaw and Kimura Techniques
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Cited by 2 •
2024
Here, we present a step-by-step protocol of robotic spleen preserving distal pancreatectomy, with and without preserving the splenic vessels (i.e., the Kimura and Warshaw techniques, respectively).
02:03
Assessing Bacterial Burden in the Mouse Spleen for Infection Studies
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2026
Source: Madenspacher, J. H. and Fessler, M. B. A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice. J. Vis. Exp. (2016)This video demonstrates the process of quantifying bacterial load in the spleen of an infected mouse to assess the severity of infection. The homogenized spleen is serially diluted and plated to determine total bacterial burden. The resulting data provide insights into host-pathogen interactions within...
04:47
A Mouse Model of Vascularized Heterotopic Spleen Transplantation
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2025
The video demonstrates a surgical method for generating a mouse model of vascularized heterotopic spleen transplantation. The abdominal cavity of an anesthetized donor mouse is surgically opened to remove the spleen. The inferior vena cava and infrarenal aorta are identified, their lumbar branches are ligated, and openings are made for the heterotopic transplantation of a spleen from a congenic donor mouse. Upon completing vascular anastomosis and restoring blood flow to the implanted spleen,...
05:18
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Cited by 22 •
2008
This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.
03:52
Quantification of Bacterial Load in Mouse Spleen and Liver After Systemic Infection
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2026
Source: Ahn, J. J., et al. Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses. J. Vis. Exp. (2016)The video demonstrates bacterial load measurement in spleen and liver tissues isolated from mice infected with an intracellular bacterial pathogen. Bacteria, accumulated within tissue-resident phagocytes during systemic infection, are released using detergent-based lysis and mechanical disruption. Serial dilutions of the homogenates are plated on...
FAQs
Surgeons may preserve perfusion through the splenic artery and vein, which requires careful separation of pancreatic tissue from adjacent splenic structures. In selected cases, collateral vessels can support splenic circulation instead. Choosing and protecting an adequate vascular route is central to maintaining splenic viability while the diseased pancreatic segment is removed.
Keeping the spleen can preserve its function and avoid the long-term risks associated with asplenia, meaning the absence of a spleen. This potential benefit explains why surgeons consider splenic preservation when disease location and oncologic requirements allow it. The decision therefore balances the value of retained splenic function against the technical demands of vascular preservation.
The approach is mainly considered for localized benign, borderline, or low-grade tumors and other disease in which oncologic principles permit spleen retention. Lesion type and biological behavior are therefore important selection factors. When preserving the spleen would conflict with appropriate cancer treatment, the technique may not be suitable, even if technically possible.
The procedure requires controlled separation of pancreatic tissue from nearby splenic structures while protecting the vessels that sustain splenic blood flow. Surgeons must also manage the remaining pancreatic tissue during distal pancreatectomy. These combined vascular and pancreatic challenges make careful dissection important for achieving disease removal without compromising the retained spleen.
Surgeons may favor splenic preservation when a localized pancreatic lesion can be treated adequately without violating oncologic principles. This is especially relevant for benign, borderline, or low-grade disease. Retaining the spleen can preserve splenic function and reduce risks linked to asplenia, making it a selective alternative rather than a universal replacement for splenectomy.
The intended outcome is removal of the diseased pancreatic portion while maintaining viable splenic tissue and its blood supply. Successful preservation may retain splenic function and avoid long-term consequences associated with splenic loss. Achieving that balance depends on suitable disease biology, localized anatomy, and effective management of both pancreatic tissue and splenic vessels.