Vickers Microhardness
Vickers Microhardness - Related Videos
08:06
A Standardized In Vitro Procedure For Evaluating Surface Microhardness Changes In Nanohybrid Resin Composites After Whey Protein Beverage Exposure
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This protocol describes a standardized in vitro method for evaluating the effects of whey protein beverages on resin composite microhardness. The approach enables controlled assessment of dietary exposure using cyclic immersion and Vickers testing, providing a reproducible model for investigating the interaction between nutritional beverages and restorative biomaterials.
06:16
Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
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Cited by 8 •
2024
Microhardness is a mechanical property and an informative parameter for evaluating hard tissue pathophysiology. Here, we demonstrate a standardized protocol (sample preparation, polishing, flat surface, and indentation sites) for microhardness analysis in tooth and alveolar bone in rodent oral disease models, namely, dental fluorosis, and ligature-induced periodontal bone resorption.
10:51
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
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A microfluidic vortex assisted electroporation platform was developed for sequential delivery of multiple molecules into identical cell populations with precise and independent dosage control. The system’s size based target cell purification step preceding electroporation aided to enhance molecular delivery efficiency and processed cell viability.
11:03
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
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Cited by 5 •
2021
Fatty acid β-oxidation is an essential metabolic pathway responsible for generating energy in many different cell types, including hepatocytes. Here, we describe a method to measure fatty acid β-oxidation in freshly isolated primary hepatocytes using 14C-labeled palmitic acid.
09:16
Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
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Cited by 12 •
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Here we describe a protocol for the preparation of agar-embedded retinal slices that are suitable for electrophysiology and Ca2+ imaging. This method allows one to study ribbon-type synapses in retinal microcircuits using direct patch-clamp recordings of single presynaptic nerve terminals.
FAQs
The impression provides a localized measure of resistance to plastic deformation. During testing, a diamond pyramid with a square base creates a controlled mark, and the two diagonal lengths quantify its size. A smaller impression for a given applied force indicates greater measured hardness, allowing small or thin specimens to be examined at the microscale.
The applied force establishes the loading condition, while the two measured diagonals characterize the resulting impression. Hardness is calculated from these quantities rather than from impression appearance alone. Consequently, comparisons require attention to both the force used and the measured mark, because their relationship determines the reported hardness value.
Its localized measurement approach helps characterize specimens that may not be suited to broader mechanical testing. Researchers can examine dental tissues, coatings, ceramics, polymers, implants, bone, and other biomaterials without treating the entire object as mechanically uniform. This microscale perspective supports comparisons of mechanical performance across diverse medical materials and tissues.
A typical measurement applies a controlled load through the square-based diamond pyramid, producing a localized impression in the specimen. The tester then measures both diagonals of that mark and uses the applied force together with impression size to calculate hardness. The resulting value can be used to compare the tested specimen with other materials or conditions.
Applications include dental enamel and dentin, bone, implants, coatings, ceramics, polymers, and other biomaterials. The method is therefore relevant to both naturally occurring tissues and engineered medical materials. By producing comparable microscale hardness measurements, it helps researchers assess how different material types perform in contexts where localized mechanical behavior matters.
Researchers can use the results to compare mechanical performance, assess treatment-related changes, and guide material optimization. Measurements may show whether a dental tissue, implant-related material, coating, ceramic, polymer, or other biomaterial has changed in localized resistance to deformation. These comparisons contribute to evaluating durability and compatibility in clinical applications.