Experimental Probe
Experimental Probe - Related Videos
07:14
Assessing Phagocytic Clearance of Cell Death in Experimental Stroke by Ligatable Fluorescent Probes
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Cited by 4 •
2014
We present a new fluorescence technique for selective in situ labeling of active phagocytic cells, which clear off cell corpses in stroke. The approach is important for assessing brain reaction to ischemia because only a small proportion of phagocytes present in ischemic brain participate in clearance of cell death.
16:01
An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging
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Cited by 4 •
2017
The development of new ultrasound (US) probes based on Capacitive Micromachined Ultrasonic Transducer (CMUT) technology requires an early realistic assessment of imaging capabilities. We describe a repeatable experimental protocol for US image acquisition and comparison with magnetic resonance images, using an ex vivo bovine brain as an imaging target.
09:49
An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
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Cited by 13 •
2018
This protocol describes the key steps for performing and analyzing pump-probe experiments combining a femtosecond optical laser with a free-electron laser in order to study ultrafast photochemical reactions in gas-phase molecules.
05:38
Custom-made Microdialysis Probe Design
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Cited by 9 •
2015
Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.
10:21
Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification
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2024
This manuscript describes the operational procedures and precautions to probe the potential common pathogenic mechanisms linking primary Sjogren's syndrome and lung adenocarcinoma through bioinformatics analysis and experimental verification.
FAQs
Interpretation depends on specificity and calibration. A probe should produce a measurable change or signal that can be linked to the targeted neural activity, signaling pathway, cell population, or anatomical structure. Calibration helps establish how the probe behaves under controlled conditions, reducing ambiguity when researchers compare responses and infer which biological process produced the observed result.
Recording probes, such as electrophysiological electrodes or fluorescent indicators, reveal changes in neural activity or related signals. Manipulating probes, including pharmacological compounds and optogenetic actuators, introduce a controlled intervention. Comparing the information from measurement with responses to intervention helps distinguish whether a neural process merely accompanies an outcome or contributes to producing it.
Controls provide a reference for judging whether an observed response results from the intended probe action rather than unrelated experimental conditions. Researchers compare responses under controlled conditions to assess specificity and link changes to the targeted activity, pathway, cell population, or structure. Without these comparisons, cause-and-effect conclusions become less secure, especially when a probe affects multiple biological features.
Selection begins with the feature being tested: neural activity, signaling, a cell population, or an anatomical structure. Researchers then choose a recording or intervention approach that can generate an interpretable measurement or change for that target. Controlled use and calibration are necessary so the resulting response can be compared with appropriate controls and connected to the hypothesis.
Experimental probes help researchers map circuit mechanisms, examine how neural processes relate to behavior, and test hypotheses about disease. They can also support evaluation of potential therapeutic strategies by revealing responses to targeted measurements or interventions. The usefulness of each application depends on whether the probe produces sufficiently specific, calibrated, and interpretable evidence under controlled conditions.
Depending on its design, a probe can reveal changes in electrical activity, fluorescence-associated signals, pharmacological responses, or effects of targeted neuronal manipulation. These outcomes allow researchers to compare neural responses with behavior, disease-related hypotheses, or therapeutic interventions. Interpreting them requires linking the measured or induced change to the relevant circuit, pathway, cell population, or anatomical structure.