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Assessing Phagocytic Clearance of Cell Death in Experimental Stroke by Ligatable Fluorescent Probes
JoVE Journal
Medicine
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JoVE Journal Medicine
Assessing Phagocytic Clearance of Cell Death in Experimental Stroke by Ligatable Fluorescent Probes
DOI:

07:14 min

May 27, 2014

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Chapters

  • 00:05Title
  • 01:23Tyramide Signal Amplification (TSA) System
  • 02:22Section Preparation
  • 03:38VACC TOPO-based Staining
  • 04:31TSA Signal Enhancement
  • 06:12Results: Imaging Phagocytic Clearance
  • 06:39Conclusion

Summary

Automatic Translation

We present a new fluorescence technique for selective in situ labeling of active phagocytic cells, which clear off cell corpses in stroke. The approach is important for assessing brain reaction to ischemia because only a small proportion of phagocytes present in ischemic brain participate in clearance of cell death.

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