Intracardial Perfusion
Intracardial Perfusion - Related Videos
03:18
Stimulation of the Intracardiac Nervous System to Assess Susceptibility to Arrhythmia
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2025
Source: Jungen, C., et al. Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System. J. Vis. Exp. (2018).The video demonstrates an ex vivo technique for stimulating the intracardiac nervous system to assess the heart’s susceptibility to arrhythmia. Using a perfusion apparatus, an isolated mouse heart is perfused with an oxygenated buffer. The intracardiac nervous system is then stimulated at a high frequency to induce arrhythmias.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
03:44
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
08:36
Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI
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Cited by 26 •
2014
A breast cancer brain metastasis mouse model is established with ultrasound imaging-guided intracardiac injection of MDA-MB231/Br-GFP cells. Development of multifocal intracranial metastases has been monitored longitudinally using high-resolution 9.4 T MRI.
FAQs
The wash solution first helps remove blood and debris from the vascular system, reducing material that can obscure tissue features or contribute to background signal. The subsequent fixative stabilizes tissue structure and molecular detail. Using these stages in sequence supports clearer histological and immunohistochemical evaluation of the brain and spinal cord after death.
Delivery through systemic vessels distributes the solutions throughout the body and toward the brain and spinal cord, rather than relying only on surface exposure. This circulation helps reach tissue through its vascular connections. As a result, nervous tissue can retain more consistent anatomical and molecular detail for later microscopy and cellular analysis.
Either cardiac pumping or controlled pressure can drive the wash solution and fixative through systemic vessels. Their role is to maintain fluid movement through the vascular pathway so blood, debris, and fixative reach the relevant tissues. The effectiveness of that circulation influences how uniformly the brain and spinal cord are prepared for downstream analysis.
The sequence of wash solution followed by fixative, the effectiveness of vascular fluid circulation, and the removal of residual blood all influence preparation quality. Deep anesthesia is typically used before the procedure. When these elements work effectively, tissue preservation improves, background from remaining blood decreases, and anatomical or cellular findings become more reliable.
The procedure is typically performed under deep anesthesia, followed by introduction of a wash solution into the heart. Fluid circulation removes blood and debris through the systemic vessels. A fixative is then introduced to preserve tissue structure and molecular detail. The prepared brain or spinal cord can subsequently undergo histology, immunohistochemistry, or microscopy.
Researchers would use intracardial perfusion when later analyses depend on well-preserved nervous tissue and reduced interference from residual blood. It is particularly relevant before histology, immunohistochemistry, and microscopy, where structural or cellular detail must remain interpretable. In neuroscience, the method therefore supports anatomical studies as well as evaluations requiring preserved molecular detail.