Propidium Iodide Positive Nuclei
Propidium Iodide Positive Nuclei - Related Videos
09:09
Annexin V and Propidium Iodide Labeling
0 Views •
2023
Staining with annexin V and propidium iodide (PI) provides researchers with a way to identify different types of cell death—either necrosis or apoptosis. This technique relies on two components. The first, annexin V, is a protein that binds certain phospholipids called phosphatidylserines, which normally occur only in the inner, cytoplasm-facing leaflet of a cell’s membrane, but become “flipped” to the outer leaflet during the early stages of apoptosis. The second component is the DNA-binding...
07:34
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
0 Views •
Cited by 543 •
2011
An accurate method for the assessment of cell death is described. The protocol improves upon conventional Annexin V/ propidium iodide (PI) protocols, which display up to 40% false- positive events in cell lines and primary cells from a broad range of animal models.
04:31
Differential Nuclear Staining Assay: An Assay to Determine Mitocan Cytotoxicity by Propidium Iodide and Hoechst Double Staining
0 Views •
2025
This video demonstrates a nuclear staining method to detect the cytotoxic effect of mitocan on drug-sensitive cancer cells and healthy variants. The double staining with DNA-binding dyes such as propidium iodide and Hoechst dye provides a clear distinction between the dead and live cells, thus helping to assess the cytotoxicity of the drug.
08:53
Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
0 Views •
Cited by 29 •
2017
This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.
12:19
Analysis of Cell Cycle Position in Mammalian Cells
0 Views •
Cited by 71 •
2012
Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.
FAQs
Intact plasma membranes normally exclude propidium iodide, limiting access to nuclear nucleic acids. When membranes or nuclei become compromised, the dye can enter and bind those targets, producing a measurable red fluorescent signal. Consequently, the proportion of labeled nuclei provides an indication of cellular injury or death and can reveal differences between experimental conditions.
In isolated-nuclei workflows, fluorescence indicates that the nuclei are accessible to propidium iodide. This interpretation differs from measurements in intact cells, where dye entry reflects compromised plasma-membrane exclusion. Reporting the preparation type is therefore important because a positive signal in isolated nuclei describes dye accessibility within the nuclear sample rather than membrane status in a complete cell.
Quantifying the labeled nuclei allows researchers to compare the extent of detectable cellular injury or death across experimental groups. Increased or decreased proportions can be evaluated after neurotoxic, ischemic, or protective treatments. The measurement is especially useful when the study asks whether a condition worsens damage or whether an intervention changes the resulting injury-associated fluorescence.
Fluorescence microscopy and flow cytometry can detect propidium iodide-associated red fluorescence. Microscopy supports visual assessment within tissue or cell preparations, whereas flow cytometry supports measurement across analyzed particle or cell populations. The choice depends on whether the experiment prioritizes spatial visualization or population-level quantification of labeled nuclei.
A typical workflow consists of preparing cells, tissue, or isolated nuclei, exposing the preparation to propidium iodide, and detecting red fluorescence with microscopy or flow cytometry. Researchers then quantify propidium iodide-positive nuclei and compare that measurement across experimental conditions. The same general sequence can support injury, death, toxicity, ischemia, or protection studies.
This measurement is useful when investigators need an indicator of cellular damage or death in neural samples. It can support comparisons among neurotoxic exposures, ischemic conditions, and protective treatments. By quantifying positive nuclei rather than relying only on qualitative images, researchers can evaluate how strongly each condition is associated with detectable nuclear accessibility and injury.