Single Neuron Recording
Single Neuron Recording - Related Videos
02:36
In Vivo Recording of Intracellular Signals from a Single Neuron
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2025
This video demonstrates the recording of electrical signals from single brain neurons using a fine-tipped glass micropipette and a specialized setup. The micropipette is carefully inserted into the rat's brain and is buzzed to penetrate the neuron to record internal neuronal electrical signals.
04:25
Simultaneous Eye Tracking and Single-Neuron Recording to Identify Target-Selective Neurons
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2025
The video demonstrates an assay to identify target-selective neurons via simultaneous eye-tracking and single-neuron recordings. A human participant is tasked with identifying a target object among an array of objects in a display, and the eye movement is tracked. Simultaneously, single neurons' firing rate is recorded via electrodes implanted in the brain. The data from both recordings are correlated to assess the presence of target-selective neurons.
06:55
Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals
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Cited by 3 •
2016
Bed bugs rely on olfactory receptor neurons housed in their antennal olfactory sensilla to detect semiochemicals in the environment. Utilizing single sensillum recording, we demonstrate a method to evaluate bed bug response to semiochemicals and explore the coding process involved.
05:01
Injectable Mesh Electronics for Stable Single-Neuron Recordings in a Mouse Brain
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2025
The video demonstrates the injection of mesh electronics probes into a mouse brain for stable long-term recordings at a single-neuron level. An anesthetized mouse with an exposed skull is secured on a stereotaxic frame, and the probe's mesh device region is injected into the brain through drilled holes. The probe's input-output pads are connected to a circuit, and recordings are obtained using a data acquisition system.
11:20
Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning
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Cited by 19 •
2014
Learning new stimulus-response associations engages a wide range of neural processes which are ultimately reflected in changing spike output of individual neurons. Here we describe a behavioral protocol allowing for the continuous registration of single-neuron activity while animals acquire, extinguish, and reacquire a conditioned response within a single experimental session.
FAQs
The recording configuration determines the question a study can address. Intracellular access provides membrane-voltage information, including action-potential activity, from the targeted neuron. Extracellular placement instead detects spikes from nearby cells, allowing researchers to monitor neuronal firing without measuring the full membrane voltage of one cell. Comparing these configurations helps align experimental design with the desired cellular or circuit-level signal.
Amplification makes detected electrical signals easier to analyze, while time-stamping preserves when each event occurred. Together, these processing steps organize neuronal activity as a signal with both measurable strength and temporal order. That timing is important when researchers relate firing to sensory inputs, movement, cognition, behavioral conditions, or activity recorded across brain regions.
Comparing neuronal firing across brain regions, behavioral conditions, or disease models places a single-cell signal in a broader circuit context. Differences in firing patterns can show that neural activity changes with sensory input, movement, cognition, or dysfunction. This comparative design is useful because it connects observations from individual neurons to changes in circuit operation rather than treating each recording as isolated.
A basic workflow begins by positioning a fine microelectrode inside or near the neuron of interest. The electrode detects electrical activity, after which the signal is amplified and time-stamped for analysis. The chosen placement determines whether the resulting data emphasize membrane voltage and action potentials or spikes detected from nearby cells.
Researchers apply the technique to examine how neurons respond to sensory inputs, participate in movement, and represent information during cognition. These applications link neuronal firing with a behavioral condition or task, allowing investigators to ask how cellular activity changes as an organism senses, acts, or processes information. The method therefore connects electrophysiological measurements with functional questions in neuroscience.
In disease-model studies, single-neuron recordings can reveal circuit dysfunction by enabling comparisons of neuronal firing across affected brain regions or experimental conditions. Investigators can examine whether activity differs across disease models and other behavioral or regional contexts. Such comparisons do not merely describe isolated spikes; they help identify how altered cellular activity may correspond to broader changes in neural-circuit function.