Tumor Tissue Processing
Tumor Tissue Processing - Related Videos
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
02:31
FFPE Tumor Tissue Macrodissection: A Technique to Obtain Specific Tumorous Tissue from Unstained Specimens
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2023
This video describes a method for the manual macrodissection of cancer tissue from unstained tissue specimens to obtain a specific cancer tissue. The tissue section can be processed further to obtain DNA for genome-wide studies on gastrointestinal cancers.
08:14
Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
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Cited by 21 •
2012
The identification of brain tumor initiating cells (BTICs), the rare cells within a heterogeneous tumor possessing stem cell properties, provides new insights into human brain tumor pathogenesis. We have refined specific culture conditions to enrich for BTICs, and we routinely use flow cytometry to further enrich these populations. Self-renewal assays and transcript analysis by single cell RT-PCR can subsequently be performed on these isolated cells.
02:41
Cryopreserved Tissue Section Fixation and Staining: A Procedure to Process Frozen Brain Tissue Sections for Laser Microdissection
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2023
This video presents the fixation and staining method to process frozen mouse brain tumor tissue. The processed tissue helps differentiate and obtain a specific population of cells during subsequent laser capture microdissection.
05:33
Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
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2023
Organotypic primary tissue explant cultures, which include precision-cut slices, represent the three-dimensional (3-D) tissue architecture as well as the multicellular interactions of native tissue. This video describes the culturing of murine lung tumor-derived tissue slices using a rotating incubation unit, a system that enables intermittent exposure of tissues to oxygen and nutrients. The protocol describes the methodology to generate and culture precision-cut lung tumor slices for drug...
FAQs
Preserving cellular architecture allows microscopic and pathological analyses to retain the spatial relationships present in the specimen. Those relationships can show how malignant cells are arranged relative to neurons, glial cells, blood vessels, and surrounding tissue. Maintaining this organization therefore supports interpretation of tumor features, tissue interactions, and disease-related changes rather than reducing the sample to isolated molecular signals.
Fixation and freezing provide alternative ways to stabilize a specimen before further analysis. The appropriate choice depends on which disease-related information researchers need to preserve for microscopic, molecular, or pathological study. Selecting a stabilization approach that matches the intended analysis helps maintain useful cellular architecture and biological information throughout embedding, sectioning, staining, or imaging.
Neuroscience-focused processing should preserve relationships among malignant cells, neurons, glial cells, blood vessels, and adjacent tissue. These interactions provide context for understanding how a tumor exists within the nervous system rather than as an isolated mass. Well-preserved preparations can therefore support investigations of tissue organization, tumor invasion, and the local environment surrounding the lesion.
Embedding places the stabilized specimen in a supporting medium that provides physical support during cutting. This allows researchers to produce thin sections suitable for staining, microscopic examination, or imaging while retaining tissue organization. Consistent section preparation makes cellular architecture and disease-related features easier to examine across the specimen and supports comparison between different analytical approaches.
Processed specimens provide material for microscopic, molecular, and pathological analysis, allowing researchers to examine both tissue structure and disease-related information. These complementary observations can contribute to tumor classification and biomarker assessment. In nervous system tumors, combining cellular context with molecular findings is particularly useful for relating tumor characteristics to surrounding neural and vascular structures.
Processing preserves tissue features that can be examined for relationships between malignant cells and surrounding nervous system components. Such preparations support research on how tumors interact with adjacent tissue during invasion and how disease-related features change in response to treatment. Microscopic and molecular findings can therefore be evaluated together when studying tumor behavior and therapeutic effects.
Carefully prepared tumor tissue retains cellular architecture and disease-related information needed to investigate cancer biology in the nervous system. These specimens can inform models that examine interactions among malignant cells, neurons, glial cells, blood vessels, and surrounding tissue. The resulting experimental context helps connect observed tumor properties with processes relevant to classification, invasion, and treatment response.