Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
 

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

Article DOI: 10.3791/4111
September 25th, 2012

Chapters

Summary September 25th, 2012

The identification of brain tumor initiating cells (BTICs), the rare cells within a heterogeneous tumor possessing stem cell properties, provides new insights into human brain tumor pathogenesis. We have refined specific culture conditions to enrich for BTICs, and we routinely use flow cytometry to further enrich these populations. Self-renewal assays and transcript analysis by single cell RT-PCR can subsequently be performed on these isolated cells.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter