Flow Two Cast Kit
Flow Two Cast Kit - Related Videos
08:32
Indirect Fabrication of Lattice Metals with Thin Sections Using Centrifugal Casting
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Cited by 8 •
2016
An indirect additive manufacturing method combining a 3D printing of polymers with a centrifugal casting is outlined for manufacturing 3D octet truss metals (Al and Cu alloys) having a unit cell length of 5 mm with a wall thickness of 0.5 mm.
06:42
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
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Cited by 29 •
2011
In this article, we present a simple, practical technique for cerebrovascular casting that is easy to perform and can be utilized to image the vascular tree of the adult mouse brain.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
07:36
Experimental Procedure for Warm Spinning of Cast Aluminum Components
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Cited by 4 •
2017
An experimental protocol for instrumented warm rotary forming of cast aluminum alloys employing a bespoke industrially scaled apparatus is presented. Experimental considerations including thermal and mechanical effects are discussed, as well as similitude with full-scale processing of automotive wheels.
04:41
Magnetic-Activated Cell Sorting: A Method to Isolate c-Kit Positive Cells
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2023
This video describes the technique of isolating c-kit positive cells from the bone marrow of a mouse. In the protocol, we will isolate c-kit positive cells from a transgenic mouse to validate c-Fos and Dusp1 alone or together as drug targets in leukemia.
FAQs
The chamber’s defined channels or compartments establish a repeatable path for solution movement. Researchers can introduce a pharmacological solution, circulate it at a controlled flow rate, and collect it after passage through the experimental region. This organized fluid path links delivery conditions with observed cellular or compound-level responses, making exposure conditions easier to reproduce across experiments.
Defined channels and compartments organize where solutions enter, move, and are collected within the experimental chamber. Their specified arrangement supports consistent handling of pharmacological treatments and helps researchers relate fluid movement to compound transport or cellular responses. This structural control is valuable when experiments require reproducible treatment conditions across repeated observations or screening workflows.
Controlled flow makes treatment delivery an experimental variable rather than an uncontrolled feature of the setup. By maintaining selected flow rates and treatment conditions, researchers can compare exposure-dependent effects under more consistent circumstances. This control strengthens interpretation by helping distinguish responses associated with the compound and its defined delivery conditions.
Collecting solutions after they move through the chamber provides a defined point for examining the relationship between delivery and transport. The collected material can support studies of how compounds pass through the experimental system, while the controlled exposure conditions help connect those observations with cellular responses or treatment effects.
After fabricating the chamber through the casting process, researchers can establish the intended fluid path, introduce solutions, circulate them under selected flow conditions, and collect the outflow. They can then maintain the planned treatment conditions while examining compound transport, cellular responses, or exposure-dependent effects. This sequence provides a practical framework for controlled pharmacological experiments.
The setup supports in vitro investigations of drug delivery, compound transport, cellular responses, and effects that depend on exposure conditions. It can also contribute to screening workflows in which researchers need controlled treatment and flow conditions. These applications allow pharmacological questions to be studied in an organized experimental chamber rather than through uncontrolled solution handling.
By enabling solutions to circulate through defined experimental spaces, the kit supports perfusion-oriented studies that more closely represent controlled fluid movement than simple treatment handling. In vitro pharmacology researchers can use this format to investigate how therapeutic compounds interact with biological systems under maintained flow and treatment conditions, supporting consistency in screening and response studies.