<em>In utero</em> Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

Heather Rice; Seiyam Suth; William Cavanaugh; Jilin Bai; Tracy L. Young-Pearse

doi:10.3791/2103

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In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

<em>In utero</em> Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

JoVE Journal
Neurologia

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JoVE Journal Neurologia

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

17,666 Views

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08:24 min

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October 08, 2010

DOI:

10.3791/2103-v

DOI:

10.3791/2103-v

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08:24 min

October 08, 2010

•

3 Views

Heather Rice¹, Seiyam Suth¹, William Cavanaugh¹, Jilin Bai², Tracy L. Young-Pearse¹

¹Center for Neurologic Diseases,Brigham and Woman’s Hospital and Harvard Medical School, ²Department of Physiology and Neurobiology,University of Connecticut

Podsumowanie

In utero electroporation is a valuable method for transfecting neuronal progenitor cells in vivo. Depending upon the placement of the electrodes and the developmental timepoint of electroporation, certain subsets of cortical cells can be targeted. Targeted cells can then be analyzed in vivo or in vitro for effects of genetic alteration.