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January 31, 2012
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A method to generate organotypic slices from the E12.5 murine embryonic midbrain is described. The organotypic slice cultures can be used to observe the behavior of dopaminergic neurons or other ventral midbrain neurons.
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Bodea, G. O., Blaess, S. Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro. J. Vis. Exp. (59), e3350, doi:10.3791/3350 (2012).
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