Dziennik
/
/
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
JoVE Journal
Immunologia i Infekcje
This content is Free Access.
JoVE Journal Immunologia i Infekcje
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

English

Automatycznie wygenerowane

13,390 Views

10:39 min

June 12, 2013

DOI:

10:39 min
June 12, 2013

7 Views
, , , , , ,

Podsumowanie

Automatically generated

We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.

Read Article