Fluorescence <em>in situ</em> Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Adi Kliot; Svetlana Kontsedalov; Galina Lebedev; Marina Brumin; Pakkianathan Britto Cathrin; Julio Massaharu Marubayashi; Marisa Skaljac; Eduard Belausov; Henryk Czosnek; Murad Ghanim

doi:10.3791/51030

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Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Fluorescence <em>in situ</em> Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

JoVE Journal
Immunologia i Infekcje

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JoVE Journal Immunologia i Infekcje

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

23,541 Views

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10:38 min

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February 24, 2014

DOI:

10.3791/51030-v

DOI:

10.3791/51030-v

•

10:38 min

February 24, 2014

•

23513 Views

Adi Kliot^1,2, Svetlana Kontsedalov¹, Galina Lebedev¹, Marina Brumin¹, Pakkianathan Britto Cathrin¹, Julio Massaharu Marubayashi¹, Marisa Skaljac^1,3, Eduard Belausov⁴, Henryk Czosnek², Murad Ghanim¹

¹Department of Entomology,Volcani Center ²Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem ³Department of Applied Sciences,Institute for Adriatic Crops and Karst Reclamation ⁴The Institute of Plant Sciences,Volcani Center

Podsumowanie

We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.