System hodowli całego jelita moczowego u myszy płodu do manipulacji ex vivo szlakami sygnałowymi i trójwymiarowego obrazowania na żywo rozwoju kosmków

Overview

This article describes a whole organ culture system for live imaging of developing villi in the fetal mouse intestine. Improved imaging technology facilitates three-dimensional visualization of organ development.

Key Study Components

Area of Science

• Neuroscience
• Developmental Biology
• Imaging Techniques

Background

• Whole organ cultures allow for the study of organ development ex vivo.
• Live imaging provides insights into cellular processes during development.
• Mouse embryonic intestines serve as a model for understanding intestinal development.
• Pharmacological modulation of signaling pathways can be studied in this system.

Purpose of Study

• To grow whole organ cultures of mouse embryonic intestines.
• To enable live imaging of developing intestinal structures.
• To investigate the effects of signaling pathway modulation on intestinal development.

Methods Used

• Harvesting mouse intestines from fetuses between embryonic day 12.5 to 18.5.
• Placing intestines in transwell culture or live imaging culture plates.
• Treating intestines with media containing pharmacological inhibitors or recombinant proteins.
• Imaging cultured intestines using thick viome sections or whole mount techniques.

Main Results

• Successful establishment of whole organ cultures for live imaging.
• Observation of developing villi in the fetal mouse intestine.
• Demonstration of the effects of signaling pathway modulation on intestinal development.
• Enhanced understanding of organ development through improved imaging technology.

Conclusions

• The whole organ culture system is effective for studying intestinal development.
• Live imaging techniques provide valuable insights into organogenesis.
• Modulation of signaling pathways can influence developmental processes.

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