Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Soumya Narayan; Charlee McLean; Akira Sawa; Sandra Y. Lin; Narayan Rai; MariaMananita S. Hipolito; Nicola Cascella; John J.I. Nurnberger, Jr.; Koko Ishizuka; Evaristus A. Nwulia

doi:10.3791/51853

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Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

JoVE Journal
Neurologia

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JoVE Journal Neurologia

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

9,723 Views

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08:33 min

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December 04, 2014

DOI:

10.3791/51853-v

DOI:

10.3791/51853-v

•

08:33 min

December 04, 2014

•

3 Views

Soumya Narayan¹, Charlee McLean², Akira Sawa¹, Sandra Y. Lin³, Narayan Rai², MariaMananita S. Hipolito², Nicola Cascella⁴, John J.I. Nurnberger, Jr.⁵, Koko Ishizuka¹, Evaristus A. Nwulia²

¹Department of Psychiatry,Johns Hopkins University, ²Department of Psychiatry and Behavioral Sciences,Howard University, ³Department of Otolaryngology-Head and Neck Surgery,Johns Hopkins University, ⁴Department of Psychiatry,Sheppard Pratt Hospital, ⁵Department of Psychiatry,Indiana University

Podsumowanie

In this study, a novel platform to investigate intraneuronal molecular signatures of treatment response in bipolar disorder (BD) was developed and validated. Olfactory epithelium from BD patients was obtained through nasal biopsies. Then laser-capture microdissection was combined with Real Time RT PCR to investigate the molecular signature of lithium response in BD.