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May 11, 2015
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We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.
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Do, O. H., Low, J. T., Thorn, P. Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets. J. Vis. Exp. (99), e52632, doi:10.3791/52632 (2015).
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