20,279 Views
•
12:36 min
•
January 14, 2016
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.
Powiązane Filmy
05:43
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Powiązane Filmy
8246 Views
12:29
mRNA Interactome Capture from Plant Protoplasts
Powiązane Filmy
9025 Views
07:08
Detection of Histone Modifications in Plant Leaves
Powiązane Filmy
24810 Views
08:21
Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System
Powiązane Filmy
25262 Views
07:02
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Powiązane Filmy
23794 Views
10:07
Identification of Post-translational Modifications of Plant Protein Complexes
Powiązane Filmy
23810 Views
09:33
An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
Powiązane Filmy
13115 Views
07:55
Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Powiązane Filmy
13807 Views
08:36
AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
Powiązane Filmy
1567 Views
06:29
Test immunoprecypitacji chromatyny: technika badania interakcji białko-DNA w komórkach
Powiązane Filmy
1407 Views
Read Article
Cytuj ten artykuł
Komar, D. N., Mouriz, A., Jarillo, J. A., Piñeiro, M. Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo. J. Vis. Exp. (107), e53422, doi:10.3791/53422 (2016).
Copy