11,917 Views
•
09:48 min
•
July 27, 2016
DOI:
Podsumowanie
Automatically generated
MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.
Powiązane Filmy
10:39
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
Powiązane Filmy
11317 Views
07:27
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
Powiązane Filmy
20554 Views
11:13
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
Powiązane Filmy
8730 Views
06:47
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Powiązane Filmy
11069 Views
06:59
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Powiązane Filmy
10286 Views
10:45
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
Powiązane Filmy
31812 Views
04:41
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Powiązane Filmy
8089 Views
06:44
Isolation of Small Noncoding RNAs from Human Serum
Powiązane Filmy
18093 Views
14:15
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
Powiązane Filmy
11869 Views
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
Powiązane Filmy
1266 Views
Read Article
Cytuj ten artykuł
Seneshaw, M., Mirshahi, F., Min, H., Asgharpour, A., Mirshahi, S., Daita, K., Boyett, S., Santhekadur, P. K., Fuchs, M., Sanyal, A. J. Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein. J. Vis. Exp. (113), e54257, doi:10.3791/54257 (2016).
Copy